Team:TU Delft/16 July 2010 content

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(Characterization of Anderson RBS sequences)
(Characterization of Anderson RBS sequences)
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Data analysis to follow shortly (good stuff!)
Data analysis to follow shortly (good stuff!)
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<h5>Assembly of reference construct & Positive control</h5>
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<h5>Assembly of reference construct & positive control</h5>
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[http://2010.igem.org/Team:TU_Delft#/blog?blog=15_July_2010 Yesterday's] digestion products of K081005 and I13401 were set for ligation over the weekend.
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The [[Team:TU_Delft/protocols/birnboim_plasmid_isolation|Birnboim method]] was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. The following concentrations of plasmid were obtained:
The [[Team:TU_Delft/protocols/birnboim_plasmid_isolation|Birnboim method]] was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. The following concentrations of plasmid were obtained:
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Revision as of 22:48, 19 July 2010

Contents

Lab work

Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing

The colony PCR of yesterday was put on1% agarose gel

We harvested the 1 mL bacterial cells of possible correct transformations. The pellets were stored at -20 °C. We used 3 mL of the bacterial cells to make -80 °C stocks.

Characterization of Anderson RBS sequences

Fluorescence measurements Attempt #2

Data analysis to follow shortly (good stuff!)

Assembly of reference construct & positive control

Yesterday's digestion products of K081005 and I13401 were set for ligation over the weekend.

The Birnboim method was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. The following concentrations of plasmid were obtained:

BioBrick Concentration (ng/μL)
J23100
I13522