Team:TU Delft/15 July 2010 content

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(New page: ==Lab work== <h4>Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing</h4> Some of transformations of [https://2010.igem.org/Team:TU_Delft#/blog?blog=14_July_2010 yesterday] containing...)
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<h4>Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing</h4>  
<h4>Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing</h4>  
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Some of transformations of [https://2010.igem.org/Team:TU_Delft#/blog?blog=14_July_2010 yesterday] containing the different ligations gave colonies (1 a 2 per plate). We picked as many colonies as possible of every plate and performed a [[Team:TU_Delft/protocols/Colony PCR|colony PCR]] to check which colonies contained the right insert. At the same time we grow them overnight in 5 mL LB medium containing the appropriate antibiotic.
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Some of transformations of [https://2010.igem.org/Team:TU_Delft#/blog?blog=14_July_2010 yesterday] containing the different ligations gave colonies (1 a 2 per plate). We picked as many colonies as possible of every plate and performed a [[Team:TU_Delft/protocols/colony PCR|colony PCR]] to check which colonies contained the right insert. At the same time we grow them overnight in 5 mL LB medium containing the appropriate antibiotic.

Revision as of 07:26, 19 July 2010

Lab work

Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing

Some of transformations of yesterday containing the different ligations gave colonies (1 a 2 per plate). We picked as many colonies as possible of every plate and performed a colony PCR to check which colonies contained the right insert. At the same time we grow them overnight in 5 mL LB medium containing the appropriate antibiotic.

Retrieved from "http://2010.igem.org/Team:TU_Delft/15_July_2010_content"