Team:TU Delft/14 July 2010 content
From 2010.igem.org
Emulsifier
By Pieter
Today I picked the colonies that grew over night from the transformation of:
Ligation | Content |
L1 | Control (just the plasmid backbone pSB1T3) |
L2 | R0011+B0032 |
L3 | OprG + B0015 |
L4 | AlnA + B0015 |
All plates contained red and white colonies. From the control plate I picked one red colony. This is probably due to the fact that we did not purified the backbone before ligation. So some plasmids reassembled.
From the other three plates I picked 5 colonies each. I tried to avoid the red ones.
The colonies were picked according to our protocol (@TODO add link to protocol) and Colony PCR was conducted. The cultures were set in the stove to grow over night at 37 C.
The PCR products were run on gel a 1% agarose gel (10 ul sample + 2 ul LB) for 1h at 100V.