Team:TU Delft/14 July 2010 content

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The PCR products were run on gel a 1% agarose gel (10 ul sample + 2 ul LB) for 1h at 100V.
The PCR products were run on gel a 1% agarose gel (10 ul sample + 2 ul LB) for 1h at 100V.
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[[Image:TU_Delft_2010-07-14_Colony_PCR.png|Thumb|Colony PCR Products|600px]]

Revision as of 13:07, 15 July 2010

Emulsifier

By Pieter

Today I picked the colonies that grew over night from the transformation of:

Ligation Content
L1 Control (just the plasmid backbone pSB1T3)
L2 R0011+B0032
L3 OprG + B0015
L4 AlnA + B0015

All plates contained red and white colonies. From the control plate I picked one red colony. This is probably due to the fact that we did not purified the backbone before ligation. So some plasmids reassembled.

From the other three plates I picked 5 colonies each. I tried to avoid the red ones.

The colonies were picked according to our protocol (@TODO add link to protocol) and Colony PCR was conducted. The cultures were set in the stove to grow over night at 37 C.

The PCR products were run on gel a 1% agarose gel (10 ul sample + 2 ul LB) for 1h at 100V.

Colony PCR Products