Team:TU Delft/13 July 2010 content

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Lab work

Emulsifier

Today the digestion products from yesterday were run on gel to see whether the plasmids were cut in the right way.

1% Agarose gel runned at 100V for 1h
EuroGentec SmartLadder

Lane description

# Description Expected Length (bp)
1 Biorad EZ marker
2 Undigested pSB1T3
3 Digested [http://partsregistry.org/Part:pSB1T3 pSB1T3] 2500
4 Undigested AlnA
5 Digested AlnA 1107
6 Undigested OprG
7 Digested OprG 744
8 Undigested B0015
9 Digested [http://partsregistry.org/Part:BBa_B0015 B0015] 130
10 Undigested R0011 !
11 Digested [http://partsregistry.org/Part:BBa_R0011 R0011] 55
12 Undigested B0032
13 Digested [http://partsregistry.org/Part:BBa_B0032 B0032] 13
14 SmartLadder

! Sample not fully loaded on gel

The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel.

The ligation products that were incubated over night were transformed to Top10 competent cells according to the protocol.