Team:TU Delft/13 July 2010 content

From 2010.igem.org

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The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel.
The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel.
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====Transformation====
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The ligation products that were incubated over night were transformed to TOP10 competent cells according to the [[Team:TU_Delft/protocols/transformation|protocol]].
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The ligation products that were incubated over night were transformed to TOP10 competent cells according to the protocol (@TODO: link!).
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Revision as of 18:18, 13 July 2010

Emulsifier

By Pieter

Today the digestion products from yesterday were run on gel to see whether the plasmids were cut in the right way.

1% Agarose gel runned at 100V for 1h
EuroGentec SmartLadder

Lane description

# Description Expected Length (bp)
1 Biorad EZ marker
2 Undigested plasmid backbone pSB1T3
3 Digested plasmid backbone pSB1T3 2500
4 Undigested AlnA
5 Digested AlnA 1107
6 Undigested OprG
7 Digested OprG 744
8 Undigested B0015
9 Digested B0015 130
10 Undigested R0011 !
11 Digested R0011 55
12 Undigested B0032
13 Digested B0032 13
14 SmartLadder

! Sample not fully loaded on gel

The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel.

The ligation products that were incubated over night were transformed to TOP10 competent cells according to the protocol.

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