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Team:TU Delft/13 July 2010 content
From 2010.igem.org
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Today the digestion products were run on gel to see whether the plasmids were cut in the right way. | Today the digestion products were run on gel to see whether the plasmids were cut in the right way. | ||
| - | [[Image:TU_Delft_2010-07-13_P_Digestion.png|600px]] | + | [[Image:TU_Delft_2010-07-13_P_Digestion.png|thumb|left|600px|1% Agarose gel runned at 100V for 1h]] |
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| + | [[Image:TU_Delft_SmartLadder.jpg|thumb|right]] | ||
Lane description | Lane description | ||
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'''!''' Sample not fully loaded on gel | '''!''' Sample not fully loaded on gel | ||
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| + | The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel. | ||
Revision as of 14:19, 13 July 2010
Emulsifier
By Pieter
Today the digestion products were run on gel to see whether the plasmids were cut in the right way.
Lane description
| # | Description | Length (bp) |
| 1 | Biorad EZ marker | |
| 2 | Undigested plasmid backbone | |
| 3 | Digested plasmid backbone pSB1T3 | 2500 |
| 4 | Undigested AlnA | |
| 5 | Digested AlnA | 1107 |
| 6 | Undigested OprG | |
| 7 | Digested OprG | 744 |
| 8 | Undigested B0015 | |
| 9 | Digested B0015 | 130 |
| 10 | Undigested R0011 ! | |
| 11 | Digested R0011 | 55 |
| 12 | Undigested B0032 | |
| 13 | Digested B0032 | 13 |
| 14 | SmartLadder |
! Sample not fully loaded on gel
The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel.
