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Team:TU Delft/12 August 2010 content
From 2010.igem.org
| # | Sample | Enzyme 1 | Enzyme 2 | Enzyme 3 | Buffer | BSA | Needed fragment |
| 1 | 011A | EcoRI | SpeI | AseI | 2 (Biolabs) | ✓ | ‘E - J61100-alkB2-J61100-rubA3 - S’ |
| 2 | 013K | EcoRI | XbaI | ✗ | 3 (Biolabs) | ✓ | ‘E - J61100-rubA4-J61100-rubR-B0015-pSB1AK3 - X’ |
| 3 | 017A | EcoRI | SpeI | AseI | 2 (Biolabs) | ✓ | ‘E - J61100-ladA - S’ |
| 4 | 018A | EcoRI | XbaI | ✗ | 3 (Biolabs) | ✓ | ‘E - J61101-ADH-pSB1A2 - X’ |
| 5 | 011A | EcoRI | PstI | AseI | 3 (Biolabs) | ✓ | ‘E - J61100-alkB2-J61100-rubA3 - P’ |
| 6 | 013K | EcoRI | PstI | HindIII | 2 (Biolabs) | ✓ | ‘E - J61100-rubA4-J61100-rubR-B0015 - P’ |
| 7 | 021 | EcoRI | PstI | SmaI | 3 (Biolabs) | ✓ | ‘E - J61107-ALDH-B0015 - P’ |
| 8 | 402 | EcoRI | PstI | AseI | 3 (Biolabs) | ✓ | ‘’ |
| 9 | 403 | EcoRI | PstI | AseI | 3 (Biolabs) | ✓ | ‘’ |
| 10 | pSB1C3 | EcoRI | PstI | ✗ | 3 (Biolabs) | ✓ | ‘E - pSB1C3 - P’ |
The digestions were done during 2 hours, and checked on a gel:
[[Image:|300px|thumb|left|Digestion check]]
