Team:TU Delft/11 June 2010
From 2010.igem.org
Lab workBioBrick stocksToday we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks. The following plasmid concentrations were obtained:
Characterization of Anderson RBS sequencesNext to that we also started digesting the first parts we will be characterizing. Strain CharacterizationBy Hugo I prepared the cell bank according to the following procedure:
Now, we have a cell bank ready to be used!!! Spring WorkshopAlmost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep. |
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