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From 2010.igem.org

Alkane degradation

All of yesterday's plates contained colonies. To check whether they contain the correct insert we will do a colony PCR.

Lane description

#	Description	Primers	Expected length (bp)	✓	✗
L	SmartLadder	n/a	n/a	n/a	n/a
1-5	013A	G00100 + G00101	1929	none	all
6-10	013K	G00100 + G00101	1929	9	6,7,8,10
11-15	020A	G00100 + G00101	2482	none	all
L	SmartLadder	n/a	n/a	n/a	n/a

On the gel it looks like lane 9, containing 013K, has the correct length. We will do a plasmid isolation tomorrow. Unfortunately none of the colonies of 020A seemed to be correct. We will check some more colonies with a colony PCR overnight.