Team:Stockholm/Results/BioBricks

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Contents

Cell-penetrating peptides

Low Molecular Weight Protamine (LMWP)

Standard part

Part name:
Registry name:
Sequencing results:
Functionality: Not verified

N-part

Part name:
Registry name:
Sequencing results:
Functionality: Not verified

Transportan 10 (Tra10)

Standard part

Part name:
Registry name:
Sequencing results:
Functionality: Not verified

N-part

Part name:
Registry name:
Sequencing results:
Functionality: Not verified

Trans-Activating Transcriptional Activator (TAT)

TAT is a cell-penetrating peptide that may be used in fusions with full-length proteins to create transduction proteins with the ability to permeate the lipid bilayer of various cell types.

Purified full-length TAT fusion proteins expressed in Escherichia coli have been shown to successfully translocate into several human cell types, including all cells found in whole blood, as well as bone marrow stem cells and osteoblasts, while still retaining the fused protein's activity (Nagahara et al. 1998). The mechanism for transduction over the bilipid membrane is still a matter of debate, but has been suggested to occur through macropinocytosis, a specialized form of endocytosis (Gump and Dowdy, 2007).

Standard part

Part name: TAT cell-penetrating peptide
Registry name: BBa_K380000
Sequencing results: Verified (fasta)
Functionality: Not verified

N-part

Part name: N-part TAT cell-penetrating peptide
Registry name: BBa_K380001
Sequencing results: Verified (fasta/Blastn)
Functionality: Not verified

Proteins

Immunoglobulin G protease (IdeS)

Protein A Z-domain (ProtA-Z)

Superoxide dismutase (SOD)

Yeast Copper Chaperone (yCCS)

Basic Fibroblast Growth Factor (bFGF)