Team:Stockholm/Results/BioBricks

From 2010.igem.org

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{{Stockholm/Results}}
{{Stockholm/Results}}
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{|align="right"
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==BioBricks==
 +
===''Trans''-Activating Transcriptional Activator (TAT)===
 +
 +
====[http://partsregistry.org/Part:BBa_K380000 BBa_K380000]: Standard part====
 +
[[image:TAT_BioBrick.png]]
 +
 +
:'''Part name:''' TAT cell-penetrating peptide<br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#TAT_cell_penetrating_peptide_.28TAT.29|more]])<br />
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:Cpp1 premix.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
 +
 +
====[http://partsregistry.org/Part:BBa_K380001 BBa_K380001]: N-part====
 +
[[image:NTAT_BioBrick.png]]
 +
 +
:'''Part name:''' N-part TAT cell-penetrating peptide<br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#TAT_cell_penetrating_peptide_.28TAT.29|more]])<br />
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_12_premix.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
 +
 +
<br />
 +
----
 +
<br />
===Low Molecular Weight Protamine (LMWP)===
===Low Molecular Weight Protamine (LMWP)===
-
====Standard part====
+
====[http://partsregistry.org/Part:BBa_K380002 BBa_K380002]: Standard part)====
 +
[[image:LMWP_BioBrick.png]]
-
:'''Part name:''' <br />
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:'''Part name:''' LMWP cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Low_molecular_weight_protamine_.28LMWP.29|more]])<br />
-
:'''Registry name:''' <br />
+
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:Cpp2 premix.txt|fasta]])<br />
-
:'''Sequencing results:''' <br />
+
:'''Works:''' <span style="color:red;">Not known</span>
-
:'''Functionality:''' <span style="color:red;">Not verified</span>
+
<br />
-
====N-part====
+
====[http://partsregistry.org/Part:BBa_K380003 BBa_K380003]: N-part====
 +
[[image:nLMWP_BioBrick.png]]
-
:'''Part name:''' <br />
+
:'''Part name:''' N-part LMWP cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Low_molecular_weight_protamine_.28LMWP.29|more]])<br />
-
:'''Registry name:''' <br />
+
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_11_premix.txt|fasta]])<br />
-
:'''Sequencing results:''' <br />
+
:'''Works:''' <span style="color:red;">Not known</span>
-
:'''Functionality:''' <span style="color:red;">Not verified</span>
+
-
===Transportan 10 (Tra10)===
+
<br />
 +
----
 +
<br />
-
====Standard part====
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===Transportan 10 (Tp10)===
-
:'''Part name:''' <br />
+
====[http://partsregistry.org/Part:BBa_K380004 BBa_K380004]: Standard part====
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
[[image:Tp10_BioBrick.png]]
-
:'''Registry name:''' <br />
+
-
:'''Sequencing results:''' <br />
+
-
:'''Functionality:''' <span style="color:red;">Not verified</span>
+
-
====N-part====
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:'''Part name:''' Tp10 cell-penetrating peptide<br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Transportan_10_.28Tp10.29|more]])<br />
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Cpp12 premix.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
-
:'''Part name:''' <br />
+
====[http://partsregistry.org/Part:BBa_K380005 BBa_K380005]: N-part====
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
[[image:nTp10_BioBrick.png]]
-
:'''Registry name:''' <br />
+
-
:'''Sequencing results:''' <br />
+
-
:'''Functionality:''' <span style="color:red;">Not verified</span>
+
-
===''Trans''-Activating Transcriptional Activator (TAT)===
+
:'''Part name:''' N-part Tp10 cell-penetrating peptide<br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Transportan_10_.28Tp10.29|more]])<br />
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_5_premix.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
-
====Standard part====
+
<br />
-
[[Image:TAT_BioBrick_Stockholm2010.png|right]]
+
----
-
:'''Part name:''' TAT cell-penetrating peptide<br />
+
<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
-
:'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380000]<br />
+
-
:'''Sequencing results:''' <span style="color:green;">Verified</span> (fasta)<br />
+
-
:'''Functionality:''' <span style="color:red;">Not verified</span>
+
-
====N-part====
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===[http://partsregistry.org/Part:BBa_K380006 BBa_K380006]: Human Basic Fibroblast Growth Factor (bFGF)===
-
[[Image:NTAT_BioBrick_Stockholm2010.png|right]]
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[[image:BFGF_BioBrick.png]]
-
:'''Part name:''' N-part TAT cell-penetrating peptide<br />
+
 
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Part name:''' Human basic fibroblast growth factor, bFGF<br />
-
:'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380001]<br />
+
:'''Property:''' Growth factor ([[Team:Stockholm/Project_Idea/Proteins#Human_basic_fibroblast_growth_factor_.28bFGF.29|more]])<br />
-
:'''Sequencing results:''' <span style="color:green;">Verified</span> ([https://static.igem.org/mediawiki/2010/9/99/PSB1C3.nCCP_12_premix.txt fasta]/[https://static.igem.org/mediawiki/2010/6/6c/Blastn_pSB.N-TAT_pSB1C3.nCPP_15sep.txt Blastn])<br />
+
:'''Edited nucleotide(s):''' nt341 C &rarr; T
-
:'''Functionality:''' <span style="color:red;">Not verified</span>
+
:'''Removed restr. site(s):''' AgeI
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.bFGF_VR_premix_fasta.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
 +
 
 +
<br />
 +
----
 +
<br />
 +
 
 +
===[http://partsregistry.org/Part:BBa_K380007 BBa_K380007]: Superoxide dismutase 1 (SOD1)===
 +
[[image:SOD1_BioBrick.png]]
 +
 
 +
:'''Part name:''' Superoxide dismutase 1 protein<br />
 +
:'''Property:''' Catalyzes the reaction O<sup>-</sup><sub>2</sub> + O<sup>-</sup><sub>2</sub> + 2H<sup>+</sup> → H<sub>2</sub>O<sub>2</sub> + O<sub>2</sub> ([[Team:Stockholm/Project_Idea/Proteins#Superoxide_dismutase_1_.28SOD1.29|more]])<br />
 +
:'''Edited nucleotide(s):''' nt331 A &rarr; G
 +
:'''Removed restr. site(s):''' PfeI
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.SOD_VR_premix_fasta.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
 +
 
 +
<br />
 +
----
 +
<br />
 +
 
 +
===[http://partsregistry.org/Part:BBa_K380008 BBa_K380008]: Yeast Copper Chaperone (yCCS)===
 +
[[image:YCCS_BioBrick.png]]
-
===Immunoglobulin G protease (IdeS)===
+
:'''Part name:''' Yeast copper chaperone protein<br />
 +
:'''Property:''' Mediates the delivery of copper ions to SOD1 ([[Team:Stockholm/Project_Idea/Proteins#Yeast_copper_chaperon_.28yCCS.29|more]])<br />
 +
:'''Edited nucleotide(s):''' nt257 T &rarr; C
 +
:'''Removed restr. site(s):''' EcoRI
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.yCCS_VR_premix_fasta.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
-
===Protein A Z-domain (ProtA-Z)===
+
<br />
 +
----
 +
<br />
-
===Superoxide dismutase (SOD)===
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===[http://partsregistry.org/Part:BBa_K380009 BBa_K380009]: Protein A Z-domain (PtA-Z)===
 +
[[image:ProteinA_BioBrick.png]]
-
====Yeast Copper Chaperone (yCCS)====
+
:'''Part name:''' Protein A Z-domain<br />
 +
:'''Property:''' Immunoglobulin Fc region binding ([[Team:Stockholm/Project_Idea/Proteins#Protein_A.2C_z_domain|more]])<br />
 +
:'''Edited nucleotide(s):''' -
 +
:'''Removed restr. site(s):''' -
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.ProtA VR premix fasta.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
-
===Basic Fibroblast Growth Factor (bFGF)===
+
<br />
 +
----
 +
<br />
 +
===[http://partsregistry.org/Part:BBa_K380010 BBa_K380010]: Immunoglobulin G protease (IdeS)===
 +
[[image:IdeS_BioBrick.png]]
 +
:'''Part name:''' Immunoglobulin G protease (IdeS)<br />
 +
:'''Property:''' Immunoglobulin G protease ([[Team:Stockholm/Project_Idea/Proteins#IgG_protease_.28IdeS.29|more]])<br />
 +
:'''Edited nucleotide(s):''' -
 +
:'''Removed restr. site(s):''' -
 +
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.IgGp_VR_premix_fasta.txt|fasta]])<br />
 +
:'''Works:''' <span style="color:red;">Not known</span>
 +
|}
{{Stockholm/Footer}}
{{Stockholm/Footer}}

Latest revision as of 01:03, 28 October 2010

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↳   BioBricks Assemblies Medals criteria


Contents

BioBricks

Trans-Activating Transcriptional Activator (TAT)

BBa_K380000: Standard part

TAT BioBrick.png

Part name: TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


BBa_K380001: N-part

NTAT BioBrick.png

Part name: N-part TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known



Low Molecular Weight Protamine (LMWP)

BBa_K380002: Standard part)

LMWP BioBrick.png

Part name: LMWP cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


BBa_K380003: N-part

NLMWP BioBrick.png

Part name: N-part LMWP cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known



Transportan 10 (Tp10)

BBa_K380004: Standard part

Tp10 BioBrick.png

Part name: Tp10 cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


BBa_K380005: N-part

NTp10 BioBrick.png

Part name: N-part Tp10 cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known



BBa_K380006: Human Basic Fibroblast Growth Factor (bFGF)

BFGF BioBrick.png

Part name: Human basic fibroblast growth factor, bFGF
Property: Growth factor (more)
Edited nucleotide(s): nt341 C → T
Removed restr. site(s): AgeI
Sequenced: Yes (fasta)
Works: Not known



BBa_K380007: Superoxide dismutase 1 (SOD1)

SOD1 BioBrick.png

Part name: Superoxide dismutase 1 protein
Property: Catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2 (more)
Edited nucleotide(s): nt331 A → G
Removed restr. site(s): PfeI
Sequenced: Yes (fasta)
Works: Not known



BBa_K380008: Yeast Copper Chaperone (yCCS)

YCCS BioBrick.png

Part name: Yeast copper chaperone protein
Property: Mediates the delivery of copper ions to SOD1 (more)
Edited nucleotide(s): nt257 T → C
Removed restr. site(s): EcoRI
Sequenced: Yes (fasta)
Works: Not known



BBa_K380009: Protein A Z-domain (PtA-Z)

ProteinA BioBrick.png

Part name: Protein A Z-domain
Property: Immunoglobulin Fc region binding (more)
Edited nucleotide(s): -
Removed restr. site(s): -
Sequenced: Yes (fasta)
Works: Not known



BBa_K380010: Immunoglobulin G protease (IdeS)

IdeS BioBrick.png

Part name: Immunoglobulin G protease (IdeS)
Property: Immunoglobulin G protease (more)
Edited nucleotide(s): -
Removed restr. site(s): -
Sequenced: Yes (fasta)
Works: Not known





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

Retrieved from "http://2010.igem.org/Team:Stockholm/Results/BioBricks"