Team:Stockholm/Results/BioBricks

From 2010.igem.org

(Difference between revisions)
(→Proteins)
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:'''Part name:''' <br />
:'''Part name:''' <br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
:'''Registry name:''' <br />
:'''Registry name:''' <br />
:'''Sequencing results:''' <br />
:'''Sequencing results:''' <br />
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:'''Part name:''' <br />
:'''Part name:''' <br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
:'''Registry name:''' <br />
:'''Registry name:''' <br />
:'''Sequencing results:''' <br />
:'''Sequencing results:''' <br />
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:'''Part name:''' <br />
:'''Part name:''' <br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
:'''Registry name:''' <br />
:'''Registry name:''' <br />
:'''Sequencing results:''' <br />
:'''Sequencing results:''' <br />
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:'''Part name:''' <br />
:'''Part name:''' <br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
:'''Registry name:''' <br />
:'''Registry name:''' <br />
:'''Sequencing results:''' <br />
:'''Sequencing results:''' <br />
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===''Trans''-Activating Transcriptional Activator (TAT)===
===''Trans''-Activating Transcriptional Activator (TAT)===
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TAT is a cell-penetrating peptide that may be used in fusions with full-length proteins to create transduction proteins with the ability to permeate the lipid bilayer of various cell types.
 
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Purified full-length TAT fusion proteins expressed in ''Escherichia coli'' have been shown to successfully translocate into several human cell types, including all cells found in whole blood, as well as bone marrow stem cells and osteoblasts, while still retaining the fused protein's activity ([http://www.ncbi.nlm.nih.gov/pubmed/9846587 Nagahara ''et al.'' 1998]). The mechanism for transduction over the bilipid membrane is still a matter of debate, but has been suggested to occur through macropinocytosis, a specialized form of endocytosis ([http://www.ncbi.nlm.nih.gov/pubmed/17913584 Gump and Dowdy, 2007]).
 
====Standard part====
====Standard part====
-
 
+
[[Image:TAT_BioBrick_Stockholm2010.png|right]]
:'''Part name:''' TAT cell-penetrating peptide<br />
:'''Part name:''' TAT cell-penetrating peptide<br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
:'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380000]<br />
:'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380000]<br />
:'''Sequencing results:''' <span style="color:green;">Verified</span> (fasta)<br />
:'''Sequencing results:''' <span style="color:green;">Verified</span> (fasta)<br />
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====N-part====
====N-part====
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+
[[Image:NTAT_BioBrick_Stockholm2010.png|right]]
:'''Part name:''' N-part TAT cell-penetrating peptide<br />
:'''Part name:''' N-part TAT cell-penetrating peptide<br />
 +
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
:'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380001]<br />
:'''Registry name:''' [http://partsregistry.org/Part:BBa_K380000 BBa_K380001]<br />
:'''Sequencing results:''' <span style="color:green;">Verified</span> ([https://static.igem.org/mediawiki/2010/9/99/PSB1C3.nCCP_12_premix.txt fasta]/[https://static.igem.org/mediawiki/2010/6/6c/Blastn_pSB.N-TAT_pSB1C3.nCPP_15sep.txt Blastn])<br />
:'''Sequencing results:''' <span style="color:green;">Verified</span> ([https://static.igem.org/mediawiki/2010/9/99/PSB1C3.nCCP_12_premix.txt fasta]/[https://static.igem.org/mediawiki/2010/6/6c/Blastn_pSB.N-TAT_pSB1C3.nCPP_15sep.txt Blastn])<br />

Revision as of 00:21, 24 October 2010

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Contents

Low Molecular Weight Protamine (LMWP)

Standard part

Part name:
Property: Cell-penetrating peptide (more)
Registry name:
Sequencing results:
Functionality: Not verified

N-part

Part name:
Property: Cell-penetrating peptide (more)
Registry name:
Sequencing results:
Functionality: Not verified

Transportan 10 (Tra10)

Standard part

Part name:
Property: Cell-penetrating peptide (more)
Registry name:
Sequencing results:
Functionality: Not verified

N-part

Part name:
Property: Cell-penetrating peptide (more)
Registry name:
Sequencing results:
Functionality: Not verified

Trans-Activating Transcriptional Activator (TAT)

Standard part

TAT BioBrick Stockholm2010.png
Part name: TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Registry name: BBa_K380000
Sequencing results: Verified (fasta)
Functionality: Not verified

N-part

NTAT BioBrick Stockholm2010.png
Part name: N-part TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Registry name: BBa_K380001
Sequencing results: Verified (fasta/Blastn)
Functionality: Not verified

Immunoglobulin G protease (IdeS)

Protein A Z-domain (ProtA-Z)

Superoxide dismutase (SOD)

Yeast Copper Chaperone (yCCS)

Basic Fibroblast Growth Factor (bFGF)

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