Team:Stockholm/Project Idea/Proteins


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Superoxide dismutase 1 (SOD1)

Human soluble Superoxide dismutase 1 (SOD1) is a soluble cytoplasmic protein functional as a homodimer that binds copper and zink ions. SOD1 catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2, protecting the cell from oxidative damage. SOD1 was first cloned and expressed in Escherichia coli by Hallewell et al., (1985).

Gene (cDNA) SOD1 dimeric.png
3D structure of human SOD1 in its dimeric form. Primary citation Leinartaite et al. (2010)
Length 465 bp
Edited nucleotide(s) nt331 A → G
Removed restr. site(s) PfeI
GenBank AY450286.1
Length 154 aa
Size 15,936 Da
Fasta SOD1
First reported by Hallewell et al., (1985).

Yeast copper chaperon (yCCS)

Yeast copper chaperon protein (yCCS) is a helper chaperon specific for copper/zinc superoxide dismutase located to the cytoplasm. yCCS generates fully metallized, active SOD1 proteins that in turn protects the cell from oxidative damage.

yCCS has been shown to successfully mediate the delivery of copper ions to human SOD1 (Ahl et al. 2003). Co-expression of SOD1 and yCCS yields proteins with higher copper contents, leading to increased activity and more stable proteins.

Gene (cDNA) YSOD+yCCS interaction.jpg
3D structure of yCCS interacting with yeast superoxide dismutase (ySOD) in it's monomeric form. Ions indicated as gray orbs. Primary citation Lamb et al. 2001
Length 750 bp
Edited nucleotide(s) nt257 T → C
Removed restr. site(s) EcoRI
GenBank NM_001182535.1
Length 249 aa
Size 27,330 Da
Fasta yCCS
First reported by Culotta et al. (1997).

Human basic fibroblast growth factor (bFGF)

Gene (cDNA) BFGF.jpg
3D structure of bFGF. Primary citation Bae et al. 2010
Length 468 bp
Edited nucleotide(s) nt341 C → T
Removed restr. site(s) AgeI
Length 155 aa
Size 17,353 Da
Fasta bFGF
First reported by <unknown>

Protein A, z domain



length 174 bp
removed restriction sites -
exchanged nt -
length 58 aa


First reported by:

IgG protease, IdeS


3D structure of IdeS. Primary citation Wenig et al. 2004

Gene (cDNA)
length 930 bp
removed restriction sites -
exchanged nt -
length 339 aa
size 37,977 Da
Fasta IdeS


First reported by:

The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/