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Proteins
Superoxide dismutase 1 protein, SOD
Part:BBa_K380007
Human soluble superoxide dismutase 1 (SOD1) is a soluble cytoplasmic protein functional as a homodimer that binds copper and zink ions. SOD1 catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2, protecting the cell from oxidative damage. SOD1 was first cloned and expressed in E. coli by Hallewell et al., (1985).
Gene (cDNA)
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length
| 465 bp
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removed restriction site
| PfeI
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exchanged nt
| nt331 A → G
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Protein
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length
| 154 aa
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size
| 15,936 Da
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Fasta
| SOD1
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GenBank:AY450286.1
First reported by:Hallewell et al., (1985).
copper chaperon protein for SOD, yCCS
Part:BBa_K380008
Yeast copper chaperon protein (yCCS) is a help chaperon specific for copper/zinc superoxide dismutase located to the cytoplasm. yCCS generates fully metallized, active SOD1 proteins that in turn protects the cell from oxidative damage.
yCCS has been shown to successfully mediate the delivery of copper ions to human SOD1 (Ahl et al. 2003). Co-expression of SOD1 and yCCS yields proteins with higher copper contents, leading to increased activity and more stable proteins.
3D structure of yCCS interacting with yeast superoxide dismutase (ySOD) in it's monomeric form. Ions indicated as gray orbs. Primary citation [ Lamb et al. 2001]
Gene (cDNA)
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length
| 750bp
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removed restriction site
| EcoRI
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exchanged nt
| nt257 T → C
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Protein
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length
| 249 aa
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size
| 27,330 Da
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Fasta
| yCCS
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GenBank:NM_001182535.1
First reported by:Culotta et al. (1997).
Human basic fibroblast growth factor, bFGF
Part:BBa_K380006
Gene (cDNA)
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length
| 468 bp
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removed restriction sites
| AgeI
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exchanged nt 341 C → T
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Protein
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length
| 155 aa
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size
| 17,353 Da
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Fasta
| bFGF
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GenBank:
First reported by:
Protein A, z domain
Part:BBa_K380009
[[Image:|300px|thumb|right|]]
Genepart
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length
| 174 bp
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removed restriction sites
| -
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exchanged nt
| -
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Protein
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length
| 58 aa
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size
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Fasta
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GenBank:
First reported by:
IgG protease, IdeS
Part:BBa_K380010
Gene (cDNA)
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length
| 930 bp
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removed restriction sites
| -
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exchanged nt
| -
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Protein
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length
| 339 aa
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size
| 37,977 Da
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Fasta
| IdeS
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GenBank:
First reported by:
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