Team:Stockholm/Project Idea/Proteins
From 2010.igem.org
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== Proteins == | == Proteins == | ||
- | === Superoxide dismutase 1 | + | === Superoxide dismutase 1 (SOD1) === |
- | [http:// | + | Human soluble Superoxide dismutase 1 (SOD1) is a soluble cytoplasmic protein functional as a homodimer that binds copper and zink ions. SOD1 catalyzes the reaction O<sup>-</sup><sub>2</sub> + O<sup>-</sup><sub>2</sub> + 2H<sup>+</sup> → H<sub>2</sub>O<sub>2</sub> + O<sub>2</sub>, protecting the cell from oxidative damage. SOD1 was first cloned and expressed in ''Escherichia coli'' by [http://www.ncbi.nlm.nih.gov/pubmed/3889846 Hallewell ''et al''., (1985)]. |
- | + | {|border="1" align="center" cellpadding="3" cellspacing="0" | |
- | + | !colspan="2"|Gene (cDNA) | |
- | + | |rowspan="10" width="250"|[[Image:SOD1_dimeric.png|250px]]<br />3D structure of human SOD1 in its dimeric form. Primary citation [http://www.ncbi.nlm.nih.gov/pubmed/20822138 Leinartaite ''et al''. (2010)] | |
- | [[Image:SOD1_dimeric.png| | + | |
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- | | | + | |width="130"|'''Length''' |
- | | 465 bp | + | |width="130"|465 bp |
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- | | | + | |'''Edited nucleotide(s)''' |
- | | | + | |nt331 A → G |
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- | | | + | |'''Removed restr. site(s)''' |
- | | | + | |PfeI |
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- | | ''' | + | |'''GenBank''' |
- | | | + | |[http://www.ncbi.nlm.nih.gov/nucleotide/38489879?report=genbank&log$=nucltop&blast_rank=22&RID=CAM83NYN01S AY450286.1]<br /> |
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- | | | + | !colspan="2"|Protein |
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|- | |- | ||
- | | | + | |'''Length''' |
- | | | + | |154 aa |
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- | | Fasta | + | |'''Size''' |
- | | [http://www.ncbi.nlm.nih.gov/protein/49456443?report=fasta SOD1] | + | |15,936 Da |
+ | |- | ||
+ | |'''Fasta''' | ||
+ | |[http://www.ncbi.nlm.nih.gov/protein/49456443?report=fasta SOD1]<br /> | ||
+ | |- | ||
+ | |colspan="2"|First reported by [http://www.ncbi.nlm.nih.gov/pubmed/3889846 Hallewell ''et al''., (1985)]. | ||
|} | |} | ||
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- | Yeast copper chaperon protein (yCCS) is a | + | === Yeast copper chaperon (yCCS) === |
+ | Yeast copper chaperon protein (yCCS) is a helper chaperon specific for copper/zinc superoxide dismutase located to the cytoplasm. yCCS generates fully metallized, active SOD1 proteins that in turn protects the cell from oxidative damage. | ||
yCCS has been shown to successfully mediate the delivery of copper ions to human SOD1 ([http://www.ncbi.nlm.nih.gov/pubmed/15358352 Ahl ''et al''. 2003]). Co-expression of SOD1 and yCCS yields proteins with higher copper contents, leading to increased activity and more stable proteins. | yCCS has been shown to successfully mediate the delivery of copper ions to human SOD1 ([http://www.ncbi.nlm.nih.gov/pubmed/15358352 Ahl ''et al''. 2003]). Co-expression of SOD1 and yCCS yields proteins with higher copper contents, leading to increased activity and more stable proteins. | ||
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- | + | {|border="1" align="center" cellpadding="3" cellspacing="0" | |
- | + | !colspan="2"|Gene (cDNA) | |
- | {| | + | |rowspan="10" width="250"|[[Image:YSOD+yCCS_interaction.jpg|250px]]<br />3D structure of yCCS interacting with yeast superoxide dismutase (ySOD) in it's monomeric form. Ions indicated as gray orbs. Primary citation [http://www.ncbi.nlm.nih.gov/pubmed/11524675 Lamb ''et al''. 2001] |
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|- | |- | ||
- | | | + | |width="130"|'''Length''' |
- | | | + | |width="130"|750 bp |
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- | | | + | |'''Edited nucleotide(s)''' |
- | | | + | |nt257 T → C |
|- | |- | ||
- | | | + | |'''Removed restr. site(s)''' |
- | | | + | |EcoRI |
|- | |- | ||
- | | ''' | + | |'''GenBank''' |
- | | | + | |[http://www.ncbi.nlm.nih.gov/nuccore/NM_001182535.1?report=genbank&log$=seqview NM_001182535.1]<br /> |
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- | | | + | !colspan="2"|Protein |
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|- | |- | ||
- | | | + | |'''Length''' |
- | | | + | |249 aa |
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- | | Fasta | + | |'''Size''' |
- | | [http://www.ncbi.nlm.nih.gov/protein/596088?report=fasta yCCS] | + | |27,330 Da |
+ | |- | ||
+ | |'''Fasta''' | ||
+ | |[http://www.ncbi.nlm.nih.gov/protein/596088?report=fasta yCCS]<br /> | ||
+ | |- | ||
+ | |colspan="2"|First reported by [http://www.ncbi.nlm.nih.gov/pubmed/9295278 Culotta ''et al''. (1997)].<br /> | ||
|} | |} | ||
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- | === Human basic fibroblast growth factor | + | === Human basic fibroblast growth factor (bFGF) === |
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- | {| | + | {|border="1" align="center" cellpadding="3" cellspacing="0" |
- | | | + | !colspan="2"|Gene (cDNA) |
- | | | + | |rowspan="10" width="250"|[[Image:BFGF.jpg|250px]]<br />3D structure of bFGF. Primary citation [http://www.ncbi.nlm.nih.gov/pubmed/20133753 Bae ''et al''. 2010] |
|- | |- | ||
- | | | + | |width="130"|'''Length''' |
- | | 468 bp | + | |width="130"|468 bp |
|- | |- | ||
- | | | + | |'''Edited nucleotide(s)''' |
- | | | + | |nt341 C → T |
|- | |- | ||
- | | | + | |'''Removed restr. site(s)''' |
- | | | + | |AgeI |
|- | |- | ||
- | | ''' | + | |'''GenBank''' |
- | | | + | | <br /> |
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- | | | + | !colspan="2"|Protein |
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|- | |- | ||
- | | | + | |'''Length''' |
- | | | + | |155 aa |
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- | | Fasta | + | |'''Size''' |
- | | [http://www.ncbi.nlm.nih.gov/protein/153285461?report=fasta bFGF] | + | |17,353 Da |
+ | |- | ||
+ | |'''Fasta''' | ||
+ | |[http://www.ncbi.nlm.nih.gov/protein/153285461?report=fasta bFGF]<br /> | ||
+ | |- | ||
+ | |colspan="2"|First reported by <unknown><br /> | ||
|} | |} | ||
- | + | ---- | |
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=== Protein A, z domain === | === Protein A, z domain === |
Revision as of 07:39, 27 October 2010
ProteinsSuperoxide dismutase 1 (SOD1)Human soluble Superoxide dismutase 1 (SOD1) is a soluble cytoplasmic protein functional as a homodimer that binds copper and zink ions. SOD1 catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2, protecting the cell from oxidative damage. SOD1 was first cloned and expressed in Escherichia coli by Hallewell et al., (1985).
Yeast copper chaperon (yCCS)Yeast copper chaperon protein (yCCS) is a helper chaperon specific for copper/zinc superoxide dismutase located to the cytoplasm. yCCS generates fully metallized, active SOD1 proteins that in turn protects the cell from oxidative damage. yCCS has been shown to successfully mediate the delivery of copper ions to human SOD1 (Ahl et al. 2003). Co-expression of SOD1 and yCCS yields proteins with higher copper contents, leading to increased activity and more stable proteins.
Human basic fibroblast growth factor (bFGF)
Protein A, z domain
[[Image:|300px|thumb|right|]]
First reported by:
IgG protease, IdeS
First reported by:
|