Team:Stockholm/Modelling/Introduction

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Latest revision as of 23:39, 26 October 2010

Introduction

As a future work of our project the aim is to manipulate the bacteria to reproduce in environments outside the laboratory for bacterial therapy. Bacteria will express proteins fused to cell-penetrating peptides (CPPs) while growing on skin. Since bacteria will be outside normal lab environment, we need to choose proper assumptions to fit the situation.

In our pEX expression vector we have lacI as a repressor of the P_tac promoter, so the idea is to have a cream on the skin which contains lactose as an inducer for P_tac. It seems in this scenario we need to take into account possible details such as degradation rates, cell growth, and factors that will affect stable production such as translational and transcriptional delay. Therefore, we are aiming to achieve a predicting model for production of protein in our system of bacteria and have the model to describe how production is induced in bacteria from the time induction starts. In this model we will not include or model or try to predict re-pigmentation, because of high complications in target human cells. For further reading about mathematics one can look in this article: The mathematics of tanning J. et al. 2008.

Out of these details, there are some data we need to know in advance. These details include:

How much lactose is available for bacteria? i. e. Concentration of lactose on the skin
Production rate for biomolecules in host bacteria
Allolactose/lactose degradation rate
Allolactose/lactose absorption through skin

The Faculty of Science at Stockholm University

Swedish Vitiligo association (Svenska Vitiligoförbundet)

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 {{Stockholm/modelling}}
-The challenge that we will face for our bacteria when it comes to the production of proteins, is fusion with cpp (cell penetration peptides). Cause this could lead to the death bacteria as they will penetrate the membrane and eventually kill bacteria when they reach certain concentration. So it is crucial to have the bacteria to begin the production when it comes with contact with skin. We have LacI as repressor, so basically lactose on skin (which is possibly not harmful) will act as inducer. Moreover, a good timing is needed before CPP reaches a critical concentration to kill bacteria. It seems in this scenario we need to take into account possible details such as degradation rates.
+{|
+|[[image:SU_modelling_Icon.gif|200px|]]
+|width="600px"|
+=== Introduction ===
-Therefore, we are aiming to achieve a predicting model for production of protein in our system of bacteria and have the model to describe production in bacteria from the time bacteria is put on skin until it is dead because of CPPs penetrating bacteria membrane. In the model, we will include production of biomolecules, possible Allolactose/lactose absorption rate by skin and degradation rate in bacteria. However, we will not include or model or try predict re-pigmentation because of high complications in target human cells.
+<div align="justify">As a future work of our project the aim is to manipulate the bacteria to reproduce in environments outside the laboratory for bacterial therapy. Bacteria will express proteins fused to cell-penetrating peptides (CPPs) while growing on skin. Since bacteria will be outside normal lab environment, we need to choose proper assumptions to fit the situation.
-Out of these many details, there are some data we need to know in advance. These details include:
-Concentration of Lactose on skin
+In our pEX expression vector we have ''lacI'' as a repressor of the P<sub>tac</sub> promoter, so the idea is to have a cream on the skin which contains lactose as an inducer for P<sub>tac</sub>. It seems in this scenario we need to take into account possible details such as degradation rates, cell growth, and factors that will affect stable production such as translational and transcriptional delay. Therefore, we are aiming to achieve a predicting model for production of protein in our system of bacteria and have the model to describe how production is induced in bacteria from the time induction starts. In this model we will not include or model or try to predict re-pigmentation, because of high complications in target human cells. For further reading about mathematics one can look in this article: [http://www.biomedcentral.com/1752-0509/3/60 The mathematics of tanning J. et al. 2008].
-Production rate for biomolecules in host bacteria
-Allolactose/Lactose degradation rate
+Out of these details, there are some data we need to know in advance. These details include:
-Critical concentration of CPP
-Allolactose/Lactose absorption through skin
+:* How much lactose is available for bacteria? i. e. Concentration of lactose on the skin
+:* Production rate for biomolecules in host bacteria
+:* Allolactose/lactose degradation rate
+:* Allolactose/lactose absorption through skin
+</div>
+|}
+{{Stockholm/Footer}}