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Team:Stockholm/Introduction
From 2010.igem.org
The challenge that we will face for our bacteria when it comes to the production of proteins, is fusion with cpp (cell penetration peptides). Cause this could lead to the death bacteria as they will penetrate the membrane and eventually kill bacteria when they reach certain concentration. So it is crucial to have the bacteria to begin the production when it comes with contact with skin. We have LacI as repressor, so basically lactose on skin (which is possibly not harmful) will act as inducer. Moreover, a good timing is needed before CPP reaches a critical concentration to kill bacteria. It seems in this scenario we need to take into account possible details such as degradation rates.
Therefore, we are aiming to achieve a predicting model for production of protein in our system of bacteria and have the model to describe production in bacteria from the time bacteria is put on skin until it is dead because of CPPs penetrating bacteria membrane. In the model, we will include production of biomolecules, possible Allolactose/lactose absorption rate by skin and degradation rate in bacteria. However, we will not include or model or try predict re-pigmentation because of high complications in target human cells. Out of these many details, there are some data we need to know in advance. These details include: Concentration of Lactose on skin Production rate for biomolecules in host bacteria Allolactose/Lactose degradation rate Critical concentration of CPP Allolactose/Lactose absorption through skin
