Team:Stockholm/8 October 2010

From 2010.igem.org

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{{Stockholm/Top2}}
{{Stockholm/Top2}}
==Andreas==
==Andreas==
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[[image:Birthdaycake.jpg|Birthday cake]]
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'''Short day in lab - birthday celebrations!'''
===Growth curve assay===
===Growth curve assay===

Revision as of 12:00, 14 October 2010


Contents

Andreas

Birthday cake

Short day in lab - birthday celebrations!

Growth curve assay

Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml in 150 ml side-arm E-flasks.

Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week.

Removal of insertion in BioBrick suffixes

Plasmid prep

Spun down pSB1C3.SOD ON culture at 13,000 x g, 10 min. Supernatant discarded and cell pellet stored in -20 °C for later plasmid prep.