Team:Stockholm/8 June 2010

From 2010.igem.org

(Difference between revisions)
(New page: {{Stockholm/Top2}} = Nina = === Preparation of competent DH5α ''Escherichia coli'' cells === ''Continued from 21/6''.<br /> Prepared competent DH5α cells according to the protocol of...)
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Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.
Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.
 +
 +
----
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Absorbance:
 +
 +
* 1 ml sample from one bottle:
 +
 +
{|
 +
! Time
 +
!
 +
!
 +
!
 +
! OD
 +
|-
 +
|30min
 +
|
 +
|
 +
|
 +
|0,029
 +
|-
 +
|1h
 +
|
 +
|
 +
|
 +
|0,053
 +
|-
 +
|1h
 +
|
 +
|
 +
|
 +
|0,188
 +
|-
 +
|30min
 +
|
 +
|
 +
|
 +
|0,316
 +
 +
|}
 +
 +
*1 ml sample from two bottles:
 +
 +
{|
 +
! Time
 +
!
 +
!
 +
!
 +
! OD
 +
|-
 +
|30min
 +
|
 +
|
 +
|
 +
|0,519/0,503
 +
|-
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|7min
 +
|
 +
|
 +
|
 +
|0,578/0,566
 +
|-
 +
|5min
 +
|
 +
|
 +
|
 +
|0,598/0,600
 +
 +
 +
|}

Revision as of 20:37, 6 July 2010

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Nina

Preparation of competent DH5α Escherichia coli cells

Continued from 21/6.

Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.

Absorbance:

  • 1 ml sample from one bottle:
Time OD
30min 0,029
1h 0,053
1h 0,188
30min 0,316
  • 1 ml sample from two bottles:
Time OD
30min 0,519/0,503
7min 0,578/0,566
5min 0,598/0,600


Retrieved from "http://2010.igem.org/Team:Stockholm/8_June_2010"