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Team:Stockholm/7 June 2010
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| - | == Preparation of competent DH5α Escherichia coli cells == | + | === Preparation of competent DH5α Escherichia coli cells === |
Autoclaving bottles & solutions for next day: | Autoclaving bottles & solutions for next day: | ||
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Nina
Preparation of competent DH5α Escherichia coli cells
Autoclaving bottles & solutions for next day:
- Two 1000 ml empty bottles
- 500 ml 50 mM CaCl2 for 1000 ml cell culture
- 16 ml 50 mM CaCl2 + 15 % Glycerol
Set a 5 ml LB ON culture of DH5α in 37°C, 250 rpm.
