Team:Stockholm/7 July 2010

From 2010.igem.org

Revision as of 10:45, 26 October 2010 by JohanNordholm (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Andreas

Designed primers for some of our parts:

  • CPPs (5'-phosphorylated primers for ligation)
    • LMWP

>LMWP_N_F1
p-AATTCGCGGCCGCTTCTAGATGGTTTCTCGTCGTCGTCGTCGTCGTGG
>LMWP-CN_F2
p-TGGTCGTCGTCGTCGTACCGGTTAATACTAGTAGCGGCCGCTGCA
>LMWP_CN_R1
p-GCGGCCGCTACTAGTATTAACCGGTACGACGACGACGACCACCACGACGA
>LMWP_N_R2
p-CGACGACGACGAGAAACCATCTAGAAGCGGCCGCG
>LMWP_C_F1
p-AATTCGCGGCCGCTTCTAGATGGCCGGCGTTTCTCGTCGTCGTCGTCGTCGTGG
>LMWP_C_R2
p-CGACGACGACGAGAAACGCCGGCCATCTAGAAGCGGCCGCG

    • TAT

>TAT_N_F1
p-AATTCGCGGCCGCTTCTAGATGTACGGTCGTAAAAAACGTCGTCAGCG
>TAT_CN_F2
p-TCGTCGTACCGGTTAATACTAGTAGCGGCCGCTGCA
>TAT_CN_R1
p-GCGGCCGCTACTAGTATTAACCGGTACGACGACGCTGACGACG
>TAT_N_R2
p-TTTTTTACGACCGTACATCTAGAAGCGGCCGCG
>TAT_C_F1
p-AATTCGCGGCCGCTTCTAGATGGCCGGCTACGGTCGTAAAAAACGTCGTCAGCG
>TAT_C_R2
p-TTTTTTACGACCGTAGCCGGCCATCTAGAAGCGGCCGCG

    • Transportan 10

>Tra1_N_F1
p-AATTCGCGGCCGCTTCTAGATGGCGGGTTACCTGCTGGGTAAAATCAAC
>Tra1_CN_F2
p-CTGAAAGCGCTGGCGGCGCTGGCGAAAAAAATCCTGACCGGTTAATACTAGTAGCGGCCGCTGCA
>Tra1_CN_R1
p-GCGGCCGCTACTAGTATTAACCGGTCAGGATTTTTTTCGCCAGCGCCGCCAG
>Tra1_N_R2
p-CGCTTTCAGGTTGATTTTACCCAGCAGGTAACCCGCCATCTAGAAGCGGCCGCG
>Tra1_C_F1
p-AATTCGCGGCCGCTTCTAGATGGCCGGCGCGGGTTACCTGCTGGGTAAAATCAAC
>Tra1_C_R2
p-CGCTTTCAGGTTGATTTTACCCAGCAGGTAACCCGCGCCGGCCATCTAGAAGCGGCCGCG

  • MITF

>MITF_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCCTGGAAATGCTAGAATATAATCAC
>MITF_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTACAAGTGTGCTCCGTCTC

  • Protein A, Z domain

>ProteinA_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGTAGACAACAAATTCAACAAAGAACAAC
>ProteinA_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTTTTCGGCGCCTGAGCATCAT

  • Vitamin B9 cluster
    • FolP

>B9_FolP_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCTATGGCATCATGAATATTACGC
>B9_FolP_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTATCATTATTCGATAACCAATAGCC

    • Xtp2

>B9_Xtp2_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCACGACGACTTGGTTGATTG
>B9_Xtp2_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTAAGTTTTGTGATTAATTTTAAGGCG

    • FolC2

>B9_FolC2_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGATACAGGGACGATTGAACAACG
>B9_FolC2_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTTTGGCTTAACCCCGGCC

    • FolE

>B9_FolE_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCATTGATGAGAAGAACCAAGCAAAG
>B9_FolE_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTTGCGATTCGCTGTAAGAATTC

    • FolK

>B9_FolK_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGCAAGTAGGGAAGAACGGG
>B9_FolK_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTATCATCTAACTCACTCACATTTCTTAC

    • FolB

>B9_FolB_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGGCATGATTCGAATTAATAATTTACGCTT
>B9_FolB_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGCCATTCGGCGTCCC

  • Tyrosinase

>Tyrosinase_F
GAAGAATTCGCGGCCGCTTCTAGATGGCCGGCGCGTGGCTGGTCGGCAAG
>Tyrosinase_R
GAACTGCAGCGGCCGCTACTAGTATTAACCGGTGGCGGACACTATGGCTATTTCTAG

  • bFGF

>bFGFF
GAATCTAGATGGCCGGCGCAGCCGGGAGCATCACC
>bFGFR
GAAACTAGTATTAACCGGTGCTCTTAGCAGACATTGGAAGAAAAAGT

  • pSB1X3 verification primers

>pSB1_VF2
tgccacctgacgtctaagaa
>pSB1_VR
attaccgcctttgagtgagc





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/