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	==Andreas==		==Andreas==
		+	===Transfer of pEX.nCPP⋅SOD⋅His to BL21===
		+	====Glycerol stocks====
		+	*BL21 pEX.nLMWP⋅SOD⋅His
		+	*BL21 pEX.nTra10⋅SOD⋅His
		+
		+	===Assembly of His⋅SOD⋅cCPP constructs===
		+	====Colony PCR====
		+	#pSB1C3.His⋅SOD⋅cTra10 A-D
		+	#pSB1C3.His⋅SOD⋅cTAT A-D
		+	#pSB1C3.His⋅SOD⋅cLMWP A-D
		+
		+	Standard colony PCR settings
		+	*Elongation time: 1:30
		+
		+	====Gel verification====
		+	1 % agarose, 120 V
		+
		+	'''Expected bands'''
		+	#884 bp
		+	#854 bp
		+	#863 bp
		+
		+	'''Results'''<br />
		+	Varying band sized, with the following clones seeming correct:
		+	#A
		+	#D
		+	#B & C

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Revision as of 12:27, 5 October 2010

Contents 1 Andreas 1.1 Transfer of pEX.nCPP⋅SOD⋅His to BL21 1.1.1 Glycerol stocks 1.2 Assembly of His⋅SOD⋅cCPP constructs 1.2.1 Colony PCR 1.2.2 Gel verification

Andreas

Transfer of pEX.nCPP⋅SOD⋅His to BL21

Glycerol stocks

• BL21 pEX.nLMWP⋅SOD⋅His
• BL21 pEX.nTra10⋅SOD⋅His

Assembly of His⋅SOD⋅cCPP constructs

Colony PCR

1. pSB1C3.His⋅SOD⋅cTra10 A-D
2. pSB1C3.His⋅SOD⋅cTAT A-D
3. pSB1C3.His⋅SOD⋅cLMWP A-D

Standard colony PCR settings

• Elongation time: 1:30

Gel verification

1 % agarose, 120 V

Expected bands

1. 884 bp
2. 854 bp
3. 863 bp

Results
Varying band sized, with the following clones seeming correct:

1. A
2. D
3. B & C