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Team:Stockholm/31 August 2010
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Contents |
Mimmi
MITF-m
Gel
| well | sample |
|---|---|
| 1 | ladder |
| 2 | pSB1C3.MITF-M 8 |
| 3 | pSB1C3.MITF-M 9 |
| 4 | pSB1C3.MITF-M 10 |
| 5 | pSB1C3.MITF-M 11 |
| 6 | pSB1C3.MITF-M 12 |
| 7 | pSB1C3.MITF-M 13 |
| 8 | pSB1C3.MITF-M 14 |
| 9 | positive control |
| 10 | digested MITF (E+S) |
| 11 | digested pSB1C3 (E+S) |
Re-ligation and transformation
- Depohosphorylate vector:
| Mix | Conditions | |||
|---|---|---|---|---|
| cut pSB1C3 | 9 | time | °C | |
| A phosp | 1 | 10m | 37 | |
| tot | 10µl | 5m | 75 | |
| oo | 10 | |||
- Ligate
| Mix | (µl) | Conditions | ||
|---|---|---|---|---|
| cut pSB1C3 | 2 | time | °C | |
| cut MITF-M | 13 | 10m | 22 | |
| 5x buffer | 4 | |||
| T4 ligase | 1 | |||
| sH2O | 0 | |||
| tot | 20µl | |||
- Follow the original transformation protocol
