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Team:Stockholm/2 September 2010
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====Transformation results==== | ====Transformation results==== | ||
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Much growth on both Top10 and DH5α plates, with Top10 being slightly more competent. | Much growth on both Top10 and DH5α plates, with Top10 being slightly more competent. | ||
Revision as of 07:48, 4 September 2010
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Contents |
Andreas
His⋅SOD cloning into pMA
Transformation results
From 1/9 transformations
Much growth on both Top10 and DH5α plates, with Top10 being slightly more competent.
Colony PCR
Three clones selected for colony PCR. Standard colony PCR procedures, see protocol. Elongation time: 1 min.
- HS1
- HS2
- HS3
- Pos. control (PC): pMA.His
Gel verification
1 % agarose
