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Revision as of 21:58, 3 September 2010 (view source) AndreasConstantinou (Talk | contribs) (New page: {{Stockholm/Top2}} ==Andreas==) ← Older edit		Revision as of 06:55, 4 September 2010 (view source) AndreasConstantinou (Talk | contribs) (→Andreas) Newer edit →
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	==Andreas==		==Andreas==
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		+	===His⋅SOD cloning into pMA===
		+
		+	====Transformation results====
		+	Much growth on both Top10 and DH5α plates, with Top10 being slightly more competent.
		+
		+	====Colony PCR====
		+	Three clones selected for colony PCR. Standard colony PCR procedures, see protocol. Elongation time: 1 min.
		+	*HS1
		+	*HS2
		+	*HS3
		+	*Pos. control (PC): pMA.His
		+
		+	====Gel verification====
		+
		+	1 % agarose

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Revision as of 06:55, 4 September 2010

Contents 1 Andreas 1.1 His⋅SOD cloning into pMA 1.1.1 Transformation results 1.1.2 Colony PCR 1.1.3 Gel verification

Andreas

His⋅SOD cloning into pMA

Transformation results

Much growth on both Top10 and DH5α plates, with Top10 being slightly more competent.

Colony PCR

Three clones selected for colony PCR. Standard colony PCR procedures, see protocol. Elongation time: 1 min.

• HS1
• HS2
• HS3
• Pos. control (PC): pMA.His

Gel verification

1 % agarose