Team:Stockholm/29 September 2010

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{{Stockholm/Top2}}
{{Stockholm/Top2}}
==Andreas==
==Andreas==
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===Sequencing results===
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Sequencing results from 27/9 samples arrived and were analyzed by nucleotide BLAST.
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{|border="1" cellpadding="1" cellspacing="0"
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!Construct
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!Sequencing<br />result
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!Blastn<br />result
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!Comment
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|-
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|pEX.N-TAT&sdot;SOD&sdot;His 3
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|[[media:PEX.nTAT.SOD.his3_premix_fasta_29sep.txt|pEX.nTAT.SOD.his 3_premix]]
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|[[media:Blastn_pEX.nTAT.SOD.his_3_premix_29sep.txt‎|Blastn]]
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|'''Verified'''. Three silent mutations in His tag; does not alter a.a. sequence.
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|-
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|pEX.N-TAT&sdot;SOD&sdot;His 4
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|[[media:PEX.nTAT.SOD.his4_premix_fasta_29sep.txt‎|pEX.nTAT.SOD.his 4_premix]]
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|[[media:Blastn_pEX.nTAT.SOD.his_4_premix_29sep.txt‎|Blastn]]
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|'''<span style="color:red;">Not verified</span>'''. Incorrect sequence.
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|-
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|pSB1A2.RBS.yCCS 3
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|[[media:PSB1A2.RBS.yCCS3_premix_fasta_29sep.txt|pSB1A2.RBS.yCCS 3_premix]]
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|[[media:Blastn_pSB1A2.RBS.yCCS3_premix_29sep.txt|Blastn]]
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|'''<span style="color:orange">Partly verified</span>'''. Three mutations found: (1) Single base deletion in yCCS sequence, causing frame shift; (2) Insertion between SpeI and PstI, not affecting expression of cloning; (3) Point mutation in PstI site, disabling PstI restriction.
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|-
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|pSB1A2.RBS.yCCS 4
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|VF2
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|pA.RBS.yCCS4_VF2
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|-
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|pSB1K3.N-TAT&sdot;SOD&sdot;His 4
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|VF2
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|pK.nTAT*SH4_VF2
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|-
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|pSB1K3.N-TAT&sdot;SOD&sdot;His 5
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|VF2
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|pK.nTAT*SH5_VF2
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|-
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|pSB1K3.N-Tra10&sdot;SOD&sdot;His 5
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|VF2
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|pK.nTra10*SH5_VF2
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|-
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|pSB1K3.N-LMWP&sdot;SOD&sdot;His 1
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|VF2
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|pK.nLMWP*SH1_VF2
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|-
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|pSB1C3.N-LMWP&sdot;SOD&sdot;His 1
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|VF2
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|pC.nLMWP*SH1_VF2
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|-
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|pSB1C3.N-LMWP&sdot;SOD&sdot;His 4
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|VF2
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|pC.nLMWP*SH4_VF2
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|-
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|pEX.SOD
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|[[media:PEX.SOD_premix_fasta_29sep.txt‎|pEX.SOD_premix]]
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|[[media:Blastn_pEX.SOD_premix_29sep.txt|Blastn]]
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|'''Verified'''. Insertion found between SpeI and PstI; does not affect expression.
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|-
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|pEX.yCCS 5
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|[[media:PEX.yCCS_5_premix_fasta.txt|pEX.yCCS 5_premix]]
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|[[media:Blastn_pEX.yCCS_5_premix_29sep.txt|Blastn]]
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|'''Verified'''. Insertion found between SpeI and PstI; does not affect expression.
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|-
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|pEX.SOD&sdot;His
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|[[media:PEX.SOD.his_premix_fasta_29sep.txt|pEX.SOD.his_premix]]
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|[[media:Blastn_pEX.SOD.his_premix_29sep.txt|Blastn]]
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|'''Verified'''. Three silent mutations in His tag; does not alter a.a. sequence.
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|-
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|pEX.His&sdot;SOD
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|[[media:PEX.his.SOD_premix_29sep.txt‎|pEX.his.SOD_premix]]
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|[[media:Blastn_pEX.his.SOD_premix_29sep.txt|Blastn]]
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|'''Verified'''. Three silent mutations in His tag; does not alter a.a. sequence.
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|}

Revision as of 08:22, 29 September 2010

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Andreas

Sequencing results

Sequencing results from 27/9 samples arrived and were analyzed by nucleotide BLAST.

Construct Sequencing
result 		Blastn
result 		Comment
pEX.N-TAT⋅SOD⋅His 3 pEX.nTAT.SOD.his 3_premix Blastn Verified. Three silent mutations in His tag; does not alter a.a. sequence.
pEX.N-TAT⋅SOD⋅His 4 pEX.nTAT.SOD.his 4_premix Blastn Not verified. Incorrect sequence.
pSB1A2.RBS.yCCS 3 pSB1A2.RBS.yCCS 3_premix Blastn Partly verified. Three mutations found: (1) Single base deletion in yCCS sequence, causing frame shift; (2) Insertion between SpeI and PstI, not affecting expression of cloning; (3) Point mutation in PstI site, disabling PstI restriction.
pSB1A2.RBS.yCCS 4 VF2 pA.RBS.yCCS4_VF2
pSB1K3.N-TAT⋅SOD⋅His 4 VF2 pK.nTAT*SH4_VF2
pSB1K3.N-TAT⋅SOD⋅His 5 VF2 pK.nTAT*SH5_VF2
pSB1K3.N-Tra10⋅SOD⋅His 5 VF2 pK.nTra10*SH5_VF2
pSB1K3.N-LMWP⋅SOD⋅His 1 VF2 pK.nLMWP*SH1_VF2
pSB1C3.N-LMWP⋅SOD⋅His 1 VF2 pC.nLMWP*SH1_VF2
pSB1C3.N-LMWP⋅SOD⋅His 4 VF2 pC.nLMWP*SH4_VF2
pEX.SOD pEX.SOD_premix Blastn Verified. Insertion found between SpeI and PstI; does not affect expression.
pEX.yCCS 5 pEX.yCCS 5_premix Blastn Verified. Insertion found between SpeI and PstI; does not affect expression.
pEX.SOD⋅His pEX.SOD.his_premix Blastn Verified. Three silent mutations in His tag; does not alter a.a. sequence.
pEX.His⋅SOD pEX.his.SOD_premix Blastn Verified. Three silent mutations in His tag; does not alter a.a. sequence.
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