Team:Stockholm/29 July 2010
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===CPP troubleshooting=== | ===CPP troubleshooting=== | ||
Analyzed sequencing results of our constructed CPPs (LMWP and Transportan 10). It seems like our target vector recircularizes instead of ligating with our CPP primers. Me, Johan and Nina are working on troubleshooting this, however it looks like we will soon have to synthesize our genes, since our primer ligations just don't seem to work. | Analyzed sequencing results of our constructed CPPs (LMWP and Transportan 10). It seems like our target vector recircularizes instead of ligating with our CPP primers. Me, Johan and Nina are working on troubleshooting this, however it looks like we will soon have to synthesize our genes, since our primer ligations just don't seem to work. | ||
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+ | ===Protein expression of IgG protease from pEX=== |
Revision as of 14:42, 31 July 2010
Andreas
CPP troubleshooting
Analyzed sequencing results of our constructed CPPs (LMWP and Transportan 10). It seems like our target vector recircularizes instead of ligating with our CPP primers. Me, Johan and Nina are working on troubleshooting this, however it looks like we will soon have to synthesize our genes, since our primer ligations just don't seem to work.