Team:Stockholm/28 August 2010

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(New page: {{Stockholm/Top2}} ==Andreas==)
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{{Stockholm/Top2}}
{{Stockholm/Top2}}
==Andreas==
==Andreas==
 +
===Gel extraction===
 +
{|border="1" cellpadding="1" cellspacing="0" align="right"
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|+ align="bottom"|''No measurable DNA conc. for "Extr. Dig SOD E+A"''
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!colspan="3"|DNA concentrations
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|-
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!Sample
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!Conc [ng/μl]
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!A<sub>260</sub>/A<sub>280</sub>
 +
|-
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|Extr. N-CPP
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|align="center"|40.91
 +
|align="center"|1.69
 +
|-
 +
|Extr. Dig SOD E+A
 +
|align="center"|&ndash;
 +
|align="center"|&ndash;
 +
|-
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|Pur. Dig pMA.His E+N
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|align="center"|36.02
 +
|align="center"|1.80
 +
|}
 +
 +
''From 27/8''<br />
 +
Purified DNA from excised samples "Extr. Dig. SOD E+A" and "Extr. N-CPP cluster" using the E.Z.N.A. Gel extraction kit; procedures according to provided protocol.
 +
 +
Elution volume: 30 &mu;l (eluted twice to increase DNA yield)
 +
*Extr. N-CPP
 +
*Extr. Dig SOD E+A
 +
 +
===DNA purification of "Dig. pMA.His (E+N)"===
 +
''From 27/8''<br />
 +
DNA clean-up using the E.Z.N.A. Gel Extraction kit, following procedures for DNA purification.
 +
 +
Elution volume: 30 &mu;l (eluted twice to increase DNA yield)
 +
*Pur. Dig. pMA His E+N

Revision as of 10:43, 30 August 2010

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Andreas

Gel extraction

No measurable DNA conc. for "Extr. Dig SOD E+A"
DNA concentrations
Sample Conc [ng/μl] A260/A280
Extr. N-CPP 40.91 1.69
Extr. Dig SOD E+A – –
Pur. Dig pMA.His E+N 36.02 1.80

From 27/8
Purified DNA from excised samples "Extr. Dig. SOD E+A" and "Extr. N-CPP cluster" using the E.Z.N.A. Gel extraction kit; procedures according to provided protocol.

Elution volume: 30 μl (eluted twice to increase DNA yield)

  • Extr. N-CPP
  • Extr. Dig SOD E+A

DNA purification of "Dig. pMA.His (E+N)"

From 27/8
DNA clean-up using the E.Z.N.A. Gel Extraction kit, following procedures for DNA purification.

Elution volume: 30 μl (eluted twice to increase DNA yield)

  • Pur. Dig. pMA His E+N
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