Team:Stockholm/25 October 2010

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Mimmi

SOD activity

- continuing

mix samples blank 1 blank 3
sample solution 20
ddH2O 20 20
WST solution 200 200 200
Enzyme solution 20 20
dilution buffer 20
tot 240µl 240µl 240µl


  • Incubate in 37°C for 20 min


  • Measure A440 with nanodrop


  • SOD activity (inhibition %) = ((Ablank1 - Ablank3) - (Asample - Ablank2))/(Ablank1 - Ablank3) x 100


SOD activity2.png





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/