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Team:Stockholm/22 September 2010
From 2010.igem.org
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==Andreas== | ==Andreas== | ||
===Gel verification=== | ===Gel verification=== | ||
| + | [[image:ColPCR_newassemblies22sep.png|200px|thumb|right|'''Colony PCR gel verification of new assemblies: pSB1A2.RBS.yCCS, pEX.N-TAT*SOD*His, pSB1C3.N-LMWP*SOD*His & pSB1K3.N-LMWP*SOD*His.'''<br />4 μl λ; 4 μl sample.<br />λ = O'GeneRuler 1 kb DNA ladder.]] | ||
''Of 21/9 colony PCR samples'' | ''Of 21/9 colony PCR samples'' | ||
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'''Expected bands''' | '''Expected bands''' | ||
| - | |||
*pSB1A2.RBS⋅yCCS (pA.RBSy): 1018 bp | *pSB1A2.RBS⋅yCCS (pA.RBSy): 1018 bp | ||
| + | *pEX.N-TAT⋅SOD⋅His (pEX.nTSH): 735 bp | ||
*pSB1C3.N-LMWP⋅SOD⋅His (pC.nLSH): 857 bp | *pSB1C3.N-LMWP⋅SOD⋅His (pC.nLSH): 857 bp | ||
*pSB1K3.N-LMWP⋅SOD⋅His (pK.nLSH): 857 bp | *pSB1K3.N-LMWP⋅SOD⋅His (pK.nLSH): 857 bp | ||
| Line 14: | Line 15: | ||
'''Results''' | '''Results''' | ||
| + | *pSB1A2.RBS⋅yCCS: Seemingly correct-sized bands for '''clones 2, 3 and 4'''. | ||
| + | *pEX.N-TAT⋅SOD⋅His: Seemingly correct-sized bands for '''clones 3 & 4'''. | ||
| + | *pSB1C3.N-LMWP⋅SOD⋅His: Seemingly correct-sized bands for '''clones 1 & 4'''. | ||
| + | *pSB1K3.N-LMWP⋅SOD⋅His: Seemingly correct-sized band for '''clone 1'''. | ||
| + | |||
| + | ===ON cultures=== | ||
| + | #5 ml LB + appr. antibiotic (100 Amp, 25 Cm or 50 Km); 37 °C, 225 rpm | ||
| + | #*pSB1A2.RBS⋅yCCS: clones 2, 3 and 4 | ||
| + | #*pEX.N-TAT⋅SOD⋅His: clones 3 and 4 | ||
| + | #*pSB1C3.N-LMWP⋅SOD⋅His: clones 1 and 4 | ||
| + | #*pSB1K3.N-LMWP⋅SOD⋅His: clone 1 | ||
Revision as of 15:10, 22 September 2010
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Andreas
Gel verification
4 μl λ; 4 μl sample.
λ = O'GeneRuler 1 kb DNA ladder.
Of 21/9 colony PCR samples
1 % agarose, 120 V
Expected bands
- pSB1A2.RBS⋅yCCS (pA.RBSy): 1018 bp
- pEX.N-TAT⋅SOD⋅His (pEX.nTSH): 735 bp
- pSB1C3.N-LMWP⋅SOD⋅His (pC.nLSH): 857 bp
- pSB1K3.N-LMWP⋅SOD⋅His (pK.nLSH): 857 bp
- pSB1C3.SOD⋅His (PC): 815 bp
Results
- pSB1A2.RBS⋅yCCS: Seemingly correct-sized bands for clones 2, 3 and 4.
- pEX.N-TAT⋅SOD⋅His: Seemingly correct-sized bands for clones 3 & 4.
- pSB1C3.N-LMWP⋅SOD⋅His: Seemingly correct-sized bands for clones 1 & 4.
- pSB1K3.N-LMWP⋅SOD⋅His: Seemingly correct-sized band for clone 1.
ON cultures
- 5 ml LB + appr. antibiotic (100 Amp, 25 Cm or 50 Km); 37 °C, 225 rpm
- pSB1A2.RBS⋅yCCS: clones 2, 3 and 4
- pEX.N-TAT⋅SOD⋅His: clones 3 and 4
- pSB1C3.N-LMWP⋅SOD⋅His: clones 1 and 4
- pSB1K3.N-LMWP⋅SOD⋅His: clone 1
