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From 2010.igem.org

Andreas

IgG protease activity assay

Continued the protocol and read the wavelength at 450 nm.

Blank: 80 μl PBS + 100 μl SureBlue™ peroxidase substrate + 100 μl 1 M HCl.

Sample loading
Extract dil.	Ab dil.	1:500						1:1000
		1	2	3	4	5	6	7	8	9	10	11	12
A	0.007	0.006	0.008	0.008	0.006	0.007	0.006	0.007	0.011	0.011	0.005	0.006
B	0.096	0.099	0.054	0.056	0.060	0.062	0.035	0.033	0.047	0.049	0.048	0.050
C	0.004	0.006	0.004	0.003	0.000	–	–	–	–	–	–	–

Results

A450 measurements
	1	2	3	4	5	6	7	8	9	10	11	12
A	0.007	0.006	0.008	0.008	0.006	0.007	0.006	0.007	0.011	0.011	0.005	0.006
B	0.096	0.099	0.054	0.056	0.060	0.062	0.035	0.033	0.047	0.049	0.048	0.050
C	0.004	0.006	0.004	0.003	0.000	–	–	–	–	–	–	–