Team:Stockholm/22 October 2010

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{{Stockholm/Top2}}
{{Stockholm/Top2}}
==Andreas==
==Andreas==
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===IgG protease activity assay===
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Continued the protocol and read the wavelength at 450 nm.
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'''Blank:''' 80 μl PBS + 100 μl SureBlue™ peroxidase substrate + 100 μl 1 M HCl.
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{|border="1" cellpadding="1" cellspacing="0"
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!colspan="14"|Sample loading
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|-
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!width="50" rowspan="2"|Extract dil.
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!Ab dil.
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!colspan="6" width="300"|1:500
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!colspan="6" width="300"|1:1000
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|-
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|width="50"| 
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!width="50"|1
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!width="50"|2
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!width="50"|3
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!width="50"|4
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!width="50"|5
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!width="50"|6
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!width="50"|7
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!width="50"|8
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!width="50"|9
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!width="50"|10
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!width="50"|11
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!width="50"|12
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|-
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!A
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|align="center"|0.007
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|align="center"|0.006
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|align="center"|0.008
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|align="center"|0.008
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|align="center"|0.006
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|align="center"|0.007
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|align="center"|0.006
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|align="center"|0.007
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|align="center"|0.011
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|align="center"|0.011
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|align="center"|0.005
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|align="center"|0.006
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|-
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!B
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|align="center"|0.096
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|align="center"|0.099
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|align="center"|0.054
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|align="center"|0.056
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|align="center"|0.060
 +
|align="center"|0.062
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|align="center"|0.035
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|align="center"|0.033
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|align="center"|0.047
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|align="center"|0.049
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|align="center"|0.048
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|align="center"|0.050
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|-
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!C
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|align="center"|0.004
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|align="center"|0.006
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|align="center"|0.004
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|align="center"|0.003
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|align="center"|'''0.000'''
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|align="center"|–
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|}
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====Results====
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{|border="1" cellpadding="1" cellspacing="0"
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!colspan="13"|A<sub>450</sub> measurements
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|-
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|width="50"|&nbsp;
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!width="50"|1
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!width="50"|2
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!width="50"|3
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!width="50"|4
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!width="50"|5
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!width="50"|6
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!width="50"|7
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!width="50"|8
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!width="50"|9
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!width="50"|10
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!width="50"|11
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!width="50"|12
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|-
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!A
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|align="center"|0.007
 +
|align="center"|0.006
 +
|align="center"|0.008
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|align="center"|0.008
 +
|align="center"|0.006
 +
|align="center"|0.007
 +
|align="center"|0.006
 +
|align="center"|0.007
 +
|align="center"|0.011
 +
|align="center"|0.011
 +
|align="center"|0.005
 +
|align="center"|0.006
 +
|-
 +
!B
 +
|align="center"|0.096
 +
|align="center"|0.099
 +
|align="center"|0.054
 +
|align="center"|0.056
 +
|align="center"|0.060
 +
|align="center"|0.062
 +
|align="center"|0.035
 +
|align="center"|0.033
 +
|align="center"|0.047
 +
|align="center"|0.049
 +
|align="center"|0.048
 +
|align="center"|0.050
 +
|-
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!C
 +
|align="center"|0.004
 +
|align="center"|0.006
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|align="center"|0.004
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|align="center"|0.003
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|align="center"|'''0.000'''
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|align="center"|&ndash;
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|}

Revision as of 11:36, 22 October 2010

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Andreas

IgG protease activity assay

Continued the protocol and read the wavelength at 450 nm.

Blank: 80 μl PBS + 100 μl SureBlue™ peroxidase substrate + 100 μl 1 M HCl.

Sample loading
Extract dil. Ab dil. 1:500 1:1000
  1 2 3 4 5 6 7 8 9 10 11 12
A 0.007 0.006 0.008 0.008 0.006 0.007 0.006 0.007 0.011 0.011 0.005 0.006
B 0.096 0.099 0.054 0.056 0.060 0.062 0.035 0.033 0.047 0.049 0.048 0.050
C 0.004 0.006 0.004 0.003 0.000 – – – – – – –

Results

A450 measurements
  1 2 3 4 5 6 7 8 9 10 11 12
A 0.007 0.006 0.008 0.008 0.006 0.007 0.006 0.007 0.011 0.011 0.005 0.006
B 0.096 0.099 0.054 0.056 0.060 0.062 0.035 0.033 0.047 0.049 0.048 0.050
C 0.004 0.006 0.004 0.003 0.000 – – – – – – –
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