Team:Stockholm/21 June 2010

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== Preparation of competent Top10 Escherichia coli cells ==
== Preparation of competent Top10 Escherichia coli cells ==
* Set a 5 ml LB ON culture; 37°C, 250 rpm.
* Set a 5 ml LB ON culture; 37°C, 250 rpm.
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=Johan=
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Overnight culture of pEX
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3 falcon tubes (50 ml), 10 ml LB in each.
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24/12=2
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2*30=60µl Amp
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 +
with tip from pEX glycerol stock
{{Stockholm/Footer}}
{{Stockholm/Footer}}

Latest revision as of 18:04, 27 October 2010


Contents

Andreas

Preparation LB agar plates with antibiotics

  • 100 ug/ml Amp (20 plates)
  • 25 ug/ml Cm (20 plates)

Preparation of competent Top10 Escherichia coli cells

  • Set a 5 ml LB ON culture; 37°C, 250 rpm.

Johan

Overnight culture of pEX

3 falcon tubes (50 ml), 10 ml LB in each.

24/12=2

2*30=60µl Amp

with tip from pEX glycerol stock





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/