Team:Stockholm/21 June 2010

From 2010.igem.org

(Difference between revisions)
(Preparation LB agar plates with antibiotics)
(Andreas)
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* 100 ug/ml Amp (20 plates)
* 100 ug/ml Amp (20 plates)
* 25 ug/ml Cm (20 plates)
* 25 ug/ml Cm (20 plates)
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 +
== Preparation of competent Top10 Escherichia coli cells ==
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* Set a 5 ml LB ON culture; 37°C, 250 rpm.

Revision as of 08:53, 6 July 2010


Andreas

Preparation LB agar plates with antibiotics

  • 100 ug/ml Amp (20 plates)
  • 25 ug/ml Cm (20 plates)

Preparation of competent Top10 Escherichia coli cells

  • Set a 5 ml LB ON culture; 37°C, 250 rpm.