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Team:Stockholm/21 June 2010
From 2010.igem.org
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== Preparation of competent Top10 Escherichia coli cells == | == Preparation of competent Top10 Escherichia coli cells == | ||
* Set a 5 ml LB ON culture; 37°C, 250 rpm. | * Set a 5 ml LB ON culture; 37°C, 250 rpm. | ||
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Revision as of 10:31, 26 October 2010
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Andreas
Preparation LB agar plates with antibiotics
- 100 ug/ml Amp (20 plates)
- 25 ug/ml Cm (20 plates)
Preparation of competent Top10 Escherichia coli cells
- Set a 5 ml LB ON culture; 37°C, 250 rpm.
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