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Team:Stockholm/20 September 2010
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Andreas
Assembly of new parts
- pSB1K3.N-LMWP⋅SOD⋅His
- Dig pSB1C3.N-LMWP (E+A)
- Dig pMA.SOD⋅His (N+P)
- Dig pSB1K3.RFP (E+P)
- pSB1C3.N-LMWP⋅SOD⋅His
- Dig pSB1C3.N-LMWP (A+S)
- Dig pMA.SOD⋅His (N+S)
Digestions
| pSB1C3. N-LMWP | pMA. SOD⋅His | pSB1C3. N-LMWP | pSB1K3. N-TAT⋅SOD⋅ His 4 | |
|---|---|---|---|---|
| 10X FastDigest buffer | 3 | 3 | 3 | 2 |
| dH2O | 15.2 | 4.1 | 15.2 | 11.4 |
| DNA (1 μg) | 9.8 | 20.9 | 9.8 | 4.6 |
| AgeI | 1 | 0 | 1 | 0 |
| NgoMIV | 0 | 1 | 0 | 0 |
| FD SpeI | 1 | 1 | 0 | 0 |
| FD EcoRI | 0 | 0 | 1 | 0 |
| FD PstI | 0 | 0 | 0 | 1 |
| FD XbaI | 0 | 0 | 0 | 1 |
| 30 μl | 30 μl | 30 μl | 20 μl |
- Incubation: 37 °C, 2:00 (NgoMIV & AgeI); 0:30 (FD)
- Inactivation: 80 °C, 20 min
