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Team:Stockholm/1 October 2010
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*20 x 100 μg/ml Amp LB agar plates | *20 x 100 μg/ml Amp LB agar plates | ||
| - | ===Assembly of His⋅SOD& | + | ===Assembly of His⋅SOD⋅cCPP constructs=== |
Digested pMA.His⋅SOD constructs for later assembly into cCPP plasmids, digested by Johan. | Digested pMA.His⋅SOD constructs for later assembly into cCPP plasmids, digested by Johan. | ||
Revision as of 11:01, 4 October 2010
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Contents |
Andreas
LB agar plates
- 20 x 100 μg/ml Amp LB agar plates
Assembly of His⋅SOD⋅cCPP constructs
Digested pMA.His⋅SOD constructs for later assembly into cCPP plasmids, digested by Johan.
Digestion
| pMA.His⋅ SOD | |
|---|---|
| 10X FastDigest buffer | 3 |
| DNA (3 μg) | 7.5 |
| dH2O | 17.5 |
| FD EcoRI | 1 |
| FD AgeI | 1 |
| 30 μl |
- Incubation: 37 °C, 0:30
- Inactivation: 80 °C, 20 min
Stored in -20 °C for later ligation.
