Team:Stockholm/1 October 2010

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(New page: {{Stockholm/Top2}} ==Andreas==)
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{{Stockholm/Top2}}
{{Stockholm/Top2}}
==Andreas==
==Andreas==
 +
===LB agar plates===
 +
*20 x 100 μg/ml Amp LB agar plates
 +
 +
===Assembly of His⋅SOD&sdotcCPP constructs===
 +
Digested pMA.His⋅SOD constructs for later assembly into cCPP plasmids, digested by Johan.
 +
 +
====Digestion====
 +
{|border="1" cellpadding="1" cellspacing="0"
 +
| 
 +
!width="50"|pMA.His⋅ SOD
 +
|-
 +
|10X FastDigest buffer
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|align="center"|3
 +
|-
 +
|DNA (3 μg)
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|align="center"|7.5
 +
|-
 +
|dH<sub>2</sub>O
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|align="center"|17.5
 +
|-
 +
|FD EcoRI
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|align="center"|1
 +
|-
 +
|FD AgeI
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|align="center"|1
 +
|-
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|&nbsp;
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!30 &mu;l
 +
|}
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*Incubation: 37 &deg;C, 0:30
 +
*Inactivation: 80 &deg;C, 20 min
 +
 +
Stored in -20 &deg;C for later ligation.

Revision as of 13:52, 2 October 2010

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Contents

Andreas

LB agar plates

  • 20 x 100 μg/ml Amp LB agar plates

Assembly of His⋅SOD&sdotcCPP constructs

Digested pMA.His⋅SOD constructs for later assembly into cCPP plasmids, digested by Johan.

Digestion

  pMA.His⋅ SOD
10X FastDigest buffer 3
DNA (3 μg) 7.5
dH2O 17.5
FD EcoRI 1
FD AgeI 1
  30 μl
  • Incubation: 37 °C, 0:30
  • Inactivation: 80 °C, 20 min

Stored in -20 °C for later ligation.

Retrieved from "http://2010.igem.org/Team:Stockholm/1_October_2010"