Team:Stockholm/17 October 2010

From 2010.igem.org

(Difference between revisions)
(Gel)
Line 43: Line 43:
==== Gel ====
==== Gel ====
-
[[Image:Place_for_picture.jpg|200px|thumb|left|]]
+
[[Image:2010-10-17_pEX.SOD_operon.jpg|250px|thumb|left|]]
{|
{|
! well
! well
Line 89: Line 89:
| pC.Sh_yC 4
| pC.Sh_yC 4
|}
|}
-
 
-
 
-
 
==== new colony PCR ====
==== new colony PCR ====

Revision as of 14:51, 26 October 2010




Contents

Mimmi

clone non-CPP operon

colony PCR

mix x12 primers samples
mastermix 24.5 pEX_VF pEX.SOD.RBS.yCCS 1-4
DNA 0.5 pEX_VR pEX.SOD.his.RBS.yCCS 1-4
tot 25µl pEX.his.SOD.RBS.yCCS 1-4
positive control (pSB1C3.LMWP.SOD.his)

Gel

2010-10-17 pEX.SOD operon.jpg
well sample well sample
1 ladder 12 ladder
2 pEX.S_yC 1 13 pEX.hS_yC 1
3 pEX.S_yC 2 14 pEX.hS_yC 2
4 pEX.S_yC 3 15 pEX.hS_yC 3
5 pEX.S_yC 4 16 pEX.hS_yC 4
6 pEX.Sh_yC 1
7 pC.Sh_yC 2
8 pC.Sh_yC 3
9 pC.Sh_yC 4

new colony PCR

mix x7 primers samples
mastermix 24.5 pEX_VF pEX.SOD.his.RBS.yCCS 1-7
DNA 0.5 pEX_VR
tot 25µl


ON cultures

  • pEX.SOD.RBS.yCCS 4
  • pEX.his.SOD.RBS.yCCS 3
  • pEX.SOD
  • pEX.yCCS





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/