Team:Stockholm/13 October 2010

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(New page: {{Stockholm/Top2}} ==Andreas==)
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{{Stockholm/Top2}}
{{Stockholm/Top2}}
==Andreas==
==Andreas==
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===Plasmid prep===
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''From 12/10 ON cultures''
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{|border="1" cellpadding="1" cellspacing="0"
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!colspan="3"|DNA concentration
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|-
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!Sample
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!width="60"|Conc [ng/μl]
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!width="60"|A<sub>260</sub>/A<sub>280</sub>
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|-
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|pSB1C3.IgGp
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|align="center"|237.9
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|align="center"|1.89
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|-
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|pSB1C3.bFGF
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|align="center"|210.3
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|align="center"|1.91
 +
|-
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|pSB1C3.ProtA
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|align="center"|173.6
 +
|align="center"|1.89
 +
|-
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|pSB1C3.yCCS
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|align="center"|229.2
 +
|align="center"|1.91
 +
|-
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|pSB1C3.SOD
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|align="center"|193.0
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|align="center"|1.87
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|}
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===Sequencing===
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*15 &mu;l plasmid DNA; 1.5 &mu;l primer (VR)
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{|border="1" cellpadding="1" cellspacing="0"
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!colspan="3"|DNA concentration
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|-
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!Sample
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!width="120"|Label
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!width="120"|Sequence code
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|-
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|pSB1C3.IgGp
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|align="center"|pSB1C3.IgGp_VR
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|align="center"|ASB0045 776
 +
|-
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|pSB1C3.bFGF
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|align="center"|pSB1C3.bFGF_VR
 +
|align="center"|ASB0045 777
 +
|-
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|pSB1C3.ProtA
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|align="center"|pSB1C3.ProtA_VR
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|align="center"|ASB0045 778
 +
|-
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|pSB1C3.yCCS
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|align="center"|pSB1C3.yCCS_VR
 +
|align="center"|ASB0045 779
 +
|-
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|pSB1C3.SOD
 +
|align="center"|pSB1C3.SOD_VR
 +
|align="center"|ASB0045 780
 +
|}

Revision as of 15:51, 14 October 2010

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Andreas

Plasmid prep

From 12/10 ON cultures

DNA concentration
Sample Conc [ng/μl] A260/A280
pSB1C3.IgGp 237.9 1.89
pSB1C3.bFGF 210.3 1.91
pSB1C3.ProtA 173.6 1.89
pSB1C3.yCCS 229.2 1.91
pSB1C3.SOD 193.0 1.87

Sequencing

  • 15 μl plasmid DNA; 1.5 μl primer (VR)
DNA concentration
Sample Label Sequence code
pSB1C3.IgGp pSB1C3.IgGp_VR ASB0045 776
pSB1C3.bFGF pSB1C3.bFGF_VR ASB0045 777
pSB1C3.ProtA pSB1C3.ProtA_VR ASB0045 778
pSB1C3.yCCS pSB1C3.yCCS_VR ASB0045 779
pSB1C3.SOD pSB1C3.SOD_VR ASB0045 780
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