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Team:Stanford/NotebookPage/9 July 2010
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== Karina's Notebook == | == Karina's Notebook == | ||
| - | Today's Goal: Digest GFP, RFP, and Terminators. Will ligate (GFP + T) and (RFP + T) on monday. <br/> | + | Today's Goal: Digest GFP, RFP, and Terminators. Will ligate (GFP + T) and (RFP + T) on monday. <br/><br/> |
| - | Part #'s: | + | '''Part #'s:''' <br/> |
| - | GFP | + | GFP: E0040 (cut at S and P) <br/> |
| + | RFP: E1010 (cut at S and P) <br/> | ||
| + | Term: B1006 (cut at X and P) <br/><br/><br/> | ||
| + | |||
| + | '''Recipe''' | ||
| + | 12.0 uL DNA<br/> | ||
| + | 1.0 uL each enzyme<br/> | ||
| + | 5.0 uL NEB buffer 2<br/> | ||
| + | 5 uL BSA (10x) <br/> | ||
| + | Sterile H20 to fill up to 50 uL | ||
Revision as of 18:57, 19 July 2010
Weekly Leader Presentation: Karina
Karina's Notebook
Today's Goal: Digest GFP, RFP, and Terminators. Will ligate (GFP + T) and (RFP + T) on monday.
Part #'s:
GFP: E0040 (cut at S and P)
RFP: E1010 (cut at S and P)
Term: B1006 (cut at X and P)
Recipe
12.0 uL DNA
1.0 uL each enzyme
5.0 uL NEB buffer 2
5 uL BSA (10x)
Sterile H20 to fill up to 50 uL
