Team:Stanford/Notebook/Summaries

From 2010.igem.org

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==Timeline for Team Analog==
==Timeline for Team Analog==
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==Timeline for Team Digital==
==Timeline for Team Digital==
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*[[Team:Stanford/Notebook/Lab_Work/Week_1 | Week 1: 6-28-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_2 | Week 2: 7-05-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_3 | Week 3: 7-12-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_4 | Week 4: 7-19-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_5 | Week 5: 7-26-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_6 | Week 6: 8-02-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_7 | Week 7: 8-09-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_8 | Week 8: 8-16-2010]]
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*[[Team:Stanford/Notebook/Lab_Work/Week_9 | Week 9: 8-23-2010]]
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**
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*[[Team:Stanford/Notebook/Lab_Work/Week_10 | Week 10: 8-30-2010]]
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**NOTE: Wednesday (9-1-2010). Housing contracts expire; move out by noon.
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Revision as of 22:59, 21 July 2010

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Contents

Timeline for Team Analog

  • Week 1: 6-28-2010
    • [x] Get parts and competent cells.
    • [x] Culture parts.
    • [x] Miniprep parts/make stocks.
    • [_] Characterize parts (promoters).
  • Week 2: 7-05-2010
    • [x] Restriction digest parts.
    • [x] PCR superfolded GFP reporter.
    • [x] Gel extract parts.
    • [_] Order genes for kinases, phosphatases, and transcription factors.
    • [_] Characterize parts (promoters).
  • Week 3: 7-12-2010
    • [x] Ligate parts.
    • [x] Order primers for small parts and promoters.
    • [x] Prepare MDV presentation and get money (hopefully).
    • [x] Order genes for kinases, phosphatases, and transcription factors.
    • [_] Streak BW27783 and culture to lose its current plasmid.
    • [_] Characterize parts (promoters).
  • Week 4: 7-19-2010
    • [_] Receive primers and build promoters.
    • [_] Streak BW27783 and culture to lose its current plasmid.
    • [_] Transform ligations and conduct sequencing.
    • [_] Characterize parts (promoters).
    • [_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
    • [_] Order more primers (degradation tags, truncated proteins, etc.).
  • Week 5: 7-26-2010
    • [_] Receive protein parts and finish with assembly.
    • [_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
    • [_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)

Timeline for Team Digital










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