Team:Stanford/Notebook/Lab Work/Week 6

From 2010.igem.org

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***Heat inactivate at 65 C for 20 min
***Heat inactivate at 65 C for 20 min
*Performed first digestion (F2620 + sfGFP + pSB4k5)
*Performed first digestion (F2620 + sfGFP + pSB4k5)
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{|
+
{| align = "center"
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| Part # || Part amount || BSA || NEB (#, amount) || Enzymes (1 uL each) || Water  
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|     Part #     ||     Part amount     ||     BSA     ||     NEB (#, amount)     ||     Enzymes (1 uL each)     ||     Water  
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|-
| F2620 || 10.8 || 3 || 2, 3 || E + S || 11.2
| F2620 || 10.8 || 3 || 2, 3 || E + S || 11.2

Revision as of 17:44, 4 August 2010

Contents

8/2 Monday

Greg's Notebook

  • Inoculated freezer stocks of most of our parts with Alex
  • Began planning for promoter characterization project:
    • Going to do 3A ligations of the form: promoter + superfolded GFP + Kan plasmid
    • Process:
      • Digest parts with at least 2 ug DNA
      • PCR cleanup
      • Diagnostic gel (remember to save uncut DNA for control)
      • Ligate in PCR tubes
      • Heat inactivate at 65 C for 20 min
  • Performed first digestion (F2620 + sfGFP + pSB4k5)
Part # Part amount BSA NEB (#, amount) Enzymes (1 uL each) Water
F2620 10.8 3 2, 3 E + S 11.2
pSB4k5 20 3 3, 3 E + P 2
sfGFP 11.76 3 2, 3 X + P 10.24


8/3 Tuesday

8/4 Wednesday

8/5 Thursday

8/6 Friday