Team:Stanford/Notebook/Lab Work/Week 4

From 2010.igem.org

(Difference between revisions)
(Laura's Lab Notebook)
(Christopher's iGEM Notebook)
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==7/12 Monday==
==7/12 Monday==
-
===Christopher's iGEM Notebook===
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===Christopher's Notebook===
*Run Gel of Restricted Digested RBS+GFP+Terminators (I746908 partial)
*Run Gel of Restricted Digested RBS+GFP+Terminators (I746908 partial)
*Gel Extract
*Gel Extract

Revision as of 17:40, 20 July 2010

Quad center.jpg

Spring: Brainstorming | Spring Meetings

Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries


Contents

7/12 Monday

Christopher's Notebook

  • Run Gel of Restricted Digested RBS+GFP+Terminators (I746908 partial)
  • Gel Extract
  • Run Gel of Gel Extracted Digest Products from Friday:
 3C5     E/P 2738 bp (buffer 3)
F2620   E/S (on pSB1A2) 1061 bp
  • I746908 E/P (on psb1A2) 2093 bp-can’t separate
  • B0034 X/P (on psb1A2) 12 bp-do NOT run on gel
  • J23100 E/S (on psb1A2) 35 bp-do NOT run on gel

7/13 Tuesday

Christopher's Notebook

  • Run Gels (0.8%) of Restriction Digests:
 1M, 2M, 2J (X/P)
 F2620 (E/S)
 3C5 (E/P)
 I0500 (E/S)

Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation.

Laura's Notebook

  • restreaked plates from 7/1/10 (with Karina)
  • for each plate, pick 8 colonies and streak, starting at the center of the "pie piece," and working towards the perimeter of the plate
  • plate ID's: