Team:Stanford/Notebook/Lab Work/Week 4

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==7/12 Monday==
 +
===Christopher's iGEM Notebook===
 +
*Run Gel of Restricted Digested RBS+GFP+Terminators (I746908 partial)
 +
*Gel Extract
 +
*Run Gel of Gel Extracted Digest Products from Friday:
 +
  3C5    E/P 2738 bp (buffer 3)
 +
F2620  E/S (on pSB1A2) 1061 bp
 +
*I746908 E/P (on psb1A2) 2093 bp-can’t separate
 +
*B0034 X/P (on psb1A2)  12 bp-do NOT run on gel
 +
*J23100 E/S (on psb1A2) 35 bp-do NOT run on gel
 +
 +
==7/13 Tuesday==
 +
===Christopher's Lab Notebook for 7/13/10===
 +
*Run Gels (0.8%) of Restriction Digests:
 +
  1M, 2M, 2J (X/P)
 +
  F2620 (E/S)
 +
  3C5 (E/P)
 +
  I0500 (E/S)
 +
 +
'''Note: for PCR of I746908: after first  gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation'''.
 +
 +
 +
 +
===Laura's Lab Notebook===
 +
*restreaked plates from 7/1/10 (with Karina)
 +
*for each plate, pick 8 colonies and streak, starting at the center of the "pie piece," and working towards the perimeter of the plate
 +
*plate ID's:
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Revision as of 17:38, 20 July 2010

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Home Project Protocols Parts Notebook Team Archives

Spring: Brainstorming | Spring Meetings

Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries


Contents

7/12 Monday

Christopher's iGEM Notebook

  • Run Gel of Restricted Digested RBS+GFP+Terminators (I746908 partial)
  • Gel Extract
  • Run Gel of Gel Extracted Digest Products from Friday: 
 3C5     E/P 2738 bp (buffer 3)
F2620   E/S (on pSB1A2) 1061 bp
  • I746908 E/P (on psb1A2) 2093 bp-can’t separate
  • B0034 X/P (on psb1A2) 12 bp-do NOT run on gel
  • J23100 E/S (on psb1A2) 35 bp-do NOT run on gel

7/13 Tuesday

Christopher's Lab Notebook for 7/13/10

  • Run Gels (0.8%) of Restriction Digests: 
 1M, 2M, 2J (X/P)
 F2620 (E/S)
 3C5 (E/P)
 I0500 (E/S)

Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation.


Laura's Lab Notebook

  • restreaked plates from 7/1/10 (with Karina)
  • for each plate, pick 8 colonies and streak, starting at the center of the "pie piece," and working towards the perimeter of the plate
  • plate ID's:
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