Team:Stanford/Notebook/Lab Work/Week 1

Spring: Brainstorming | Spring Meetings

Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries

Monday

General Meeting Notes

• Wiki stuff
  • Everyone should sign up on 2010.igem.org website
    • Take advantage of the new calendar system
    • Need to transfer current website over
  • Current wiki needs a new vision
    • Francisco + Alex assembling wiki committee
• Transform the first wave of cells
  • Pick colonies
  • Colony PCR
  • Sequence parts to validate
    • Use the Smolke sequencing network
• Characterize promoters
  • Digest, ligate
  • Need to acquire enzymes
  • Need an PO account.
    • Email Jeanne
• Get competent cells
• Getting DNA
  • Assembly PCR
  • PCR from host cells
  • Synthesize de novo
• RNA team
  • Use RNAstructure
    • Maybe use ViennaRNA or dinamelt
  • Talk to Arkin and Berkeley Postdocs
  • Finalize sRNA library
  • 9kb plasmid
• Meeting scheudle
  • Monday morning is good for logistics
  • Group meetings for presentations
    • One person presents their work in the context of everything else
  • Journal club
  • Karina will set up a doodle

Tuesday

Laura: made LB agar (with Alex and Chris)

• made 1 500 mL bottle, 3 250 mL bottles, then autoclaved

Wednesday