Team:Stanford/Notebook/Lab Work/Week 1

Spring: Brainstorming | Spring Meetings

Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries

Monday

General Meeting Notes

1. Wiki stuff

• Everyone should sign up on 2010.igem.org website
  • Take advantage of the new calendar system
  • Need to transfer current website over
• Current wiki needs a new vision
  • Francisco + Alex assembling wiki committee

1. Transform the first wave of cells

• Pick colonies
• Colony PCR
• Sequence parts to validate
  • Use the Smolke sequencing network

1. Characterize promoters

• Digest, ligate
• Need to acquire enzymes
• Need an PO account.
  • Email Jeanne

1. Get competent cells
2. Getting DNA

• Assembly PCR
• PCR from host cells
• Synthesize de novo

1. RNA team

• Use RNAstructure
  • Maybe use ViennaRNA or dinamelt
• Talk to Arkin and Berkeley Postdocs
• Finalize sRNA library
• 9kb plasmid

1. Meeting scheudle

• Monday morning is good for logistics
• Group meetings for presentations
  • One person presents their work in the context of everything else
• Journal club
• Karina will set up a doodle