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Team:Stanford/Notebook/Lab Work/Week 1
From 2010.igem.org
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==Monday== | ==Monday== | ||
| - | '''General Meeting''' | + | '''General Meeting Notes''' |
| - | Wiki stuff | + | #Wiki stuff |
*Everyone should sign up on 2010.igem.org website | *Everyone should sign up on 2010.igem.org website | ||
**Take advantage of the new calendar system | **Take advantage of the new calendar system | ||
| Line 12: | Line 12: | ||
*Current wiki needs a new vision | *Current wiki needs a new vision | ||
**Francisco + Alex assembling wiki committee | **Francisco + Alex assembling wiki committee | ||
| - | Transform the first wave of cells | + | #Transform the first wave of cells |
*Pick colonies | *Pick colonies | ||
*Colony PCR | *Colony PCR | ||
*Sequence parts to validate | *Sequence parts to validate | ||
**Use the Smolke sequencing network | **Use the Smolke sequencing network | ||
| - | Characterize promoters | + | #Characterize promoters |
*Digest, ligate | *Digest, ligate | ||
*Need to acquire enzymes | *Need to acquire enzymes | ||
*Need an PO account. | *Need an PO account. | ||
**Email Jeanne | **Email Jeanne | ||
| - | Get competent cells | + | #Get competent cells |
| - | Getting DNA | + | #Getting DNA |
*Assembly PCR | *Assembly PCR | ||
*PCR from host cells | *PCR from host cells | ||
*Synthesize de novo | *Synthesize de novo | ||
| - | RNA team | + | #RNA team |
*Use RNAstructure | *Use RNAstructure | ||
**Maybe use ViennaRNA or dinamelt | **Maybe use ViennaRNA or dinamelt | ||
| Line 33: | Line 33: | ||
*Finalize sRNA library | *Finalize sRNA library | ||
*9kb plasmid | *9kb plasmid | ||
| - | Meeting scheudle | + | #Meeting scheudle |
*Monday morning is good for logistics | *Monday morning is good for logistics | ||
*Group meetings for presentations | *Group meetings for presentations | ||
Revision as of 22:40, 19 July 2010
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Spring: Brainstorming | Spring Meetings
Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries
|
Monday
General Meeting Notes
- Wiki stuff
- Everyone should sign up on 2010.igem.org website
- Take advantage of the new calendar system
- Need to transfer current website over
- Current wiki needs a new vision
- Francisco + Alex assembling wiki committee
- Transform the first wave of cells
- Pick colonies
- Colony PCR
- Sequence parts to validate
- Use the Smolke sequencing network
- Characterize promoters
- Digest, ligate
- Need to acquire enzymes
- Need an PO account.
- Email Jeanne
- Get competent cells
- Getting DNA
- Assembly PCR
- PCR from host cells
- Synthesize de novo
- RNA team
- Use RNAstructure
- Maybe use ViennaRNA or dinamelt
- Talk to Arkin and Berkeley Postdocs
- Finalize sRNA library
- 9kb plasmid
- Meeting scheudle
- Monday morning is good for logistics
- Group meetings for presentations
- One person presents their work in the context of everything else
- Journal club
- Karina will set up a doodle
