Team:Stanford/Notebook/Lab Work/Week 1

From 2010.igem.org

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(June 28)
(June 28)
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=====June 28=====
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General Meeting
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*Wiki stuff
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**Everyone should sign up on 2010.igem.org website
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***Take advantage of the new calendar system
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***Need to transfer current website over
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**Current wiki needs a new vision
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***Francisco + Alex assembling wiki committee
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*Transform the first wave of cells
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**Pick colonies
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**Colony PCR
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**Sequence parts to validate
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***Use the Smolke sequencing network
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*Characterize promoters
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**Digest, ligate
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**Need to acquire enzymes
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**Need an PO account.
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***Email Jeanne
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*Get competent cells
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*Getting DNA
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**Assembly PCR
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**PCR from host cells
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**Synthesize de novo
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*RNA team
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**Use RNAstructure
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***Maybe use ViennaRNA or dinamelt
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**Talk to Arkin and Berkeley Postdocs
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**Finalize sRNA library
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**9kb plasmid
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*Meeting scheudle
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**Monday morning is good for logistics
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**Group meetings for presentations
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***One person presents their work in the context of everything else
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**Journal club
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**Karina will set up a doodle
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Revision as of 21:30, 19 July 2010